补肾活血方治疗大鼠糖尿病性勃起功能障碍的机理

目的研究补肾活血方对糖尿病(DM)性勃起功能障碍(ED)大鼠勃起功能的疗效及其作用机理。方法将40只雄性SD大鼠随机分为正常组、糖尿病模型组、补肾活血方低剂量组、补肾活血方高剂量组,每组10只。糖尿病模型组、补肾活血方低剂量组、补肾活血方高剂量组3组大鼠腹腔注射链脲佐菌素(STZ),构建糖尿病大鼠模型。正常组和糖尿病模型组进行生理盐水灌胃,补肾活血方低剂量组(3 g/kg)和高剂量组(6 g/kg)进行补肾活血方灌胃,疗程8周,8周后取材测定阴茎脏器指数和一抗、二抗浓度,采用Biopack电生理仪检测阴茎海绵体内压(ICP),蛋白印迹法检测蛋白激酶B(AKT)、磷酸化蛋白激酶B(p AKT)表达,TUNEL法检测阴茎组织凋亡,Masson三色染色观察阴茎组织病理改变。结果与糖尿病模型组比较,补肾活血方低、高剂量组阴茎脏器指数无明显差异(P0.05),补肾活血方高剂量组ICP和ICP与平均动脉压(MAP)的比值明显升高(P0.05)。与糖尿病模型组比较,补肾活血方低、高剂量组p AKT、AKT表达显著增加(P0.05),并且阴茎海绵体平滑肌凋亡率均有所下降,但差异无显著性意义(P0.05)。补肾活血方低剂量组与糖尿病模型组相比,胶原纤维比例显著下降(P0.05)。结论补肾活血方可增加糖尿病大鼠阴茎ICP,其机理与提高p AKT表达、抑制阴茎海绵体平滑肌细胞凋亡、降低胶原纤维的生成有关。

Mechanism of invigorating kidney and activating blood recipe for the treatment of diabetic erectile dysfunction in rats

Objective To study the therapeutic effect and mechanism of invigorating kidney and activa-ting blood(IKAB)recipe,a Chinese herb compound,on diabetic erectile dysfunction (DED)in rats. Methods 40 male SD rats were randomly divided into four groups (each n =10):normal group,model group,low-dose IKAB group (low-dose group)and high-dose IKAB group(high-dose group).The rat model with 2 type of diabet mellitus was induced by intraperitoneal injection of streptozotocin (STZ). Normal group and model group were intragastrically administered (ig.)with normal saline,low-dose group was ig.3 g/kg of IKAB and high-dose group was ig.6 g/kg IKAB for consecutive 8 weeks.The penis viscera index was measured;the intracavernous pressure (ICP)were detected by Biopack electro-physiological instrument;the expressions of pAKT and AKT were detectd by Western blotting;cell apop-tosis of corpus cavernosum smooth muscle were measured by using TUNEL method and the pathological changes in penile tissue were observed by using Masson trichrome staining.Results Compared with model group,the penis viscera index of both IKAB groups had no statistical differences (P >0.05);the expressions of pAKT and AKT increased significantly(P <0.05);although the ratio of cell apoptosis of corpus cavernosum smooth muscle decreased,the statistical differences were not shown (P >0.05).Fur-thermore,ICP and ICP/MAP increased markedly in high-dose group and the collagen fiber proportion de-creased in low -dose group compared with model group (P <0.05).Conclusion The mechanism of in-vigorating kidney and activating blood recipe increasing ICP in rat model with DMis related to increasing the expression of pAKT,inhibiting cell apoptosis of corpus cavernosum smooth muscle and reducing colla-gen fibers formation in penis.