肝郁、脾虚和肝郁脾虚证模型大鼠行为学变化及柴疏四君子汤对其作用的比较

目的比较肝郁证、脾虚证和肝郁脾虚证模型大鼠的行为学变化及柴疏四君子汤对其作用的异同。方法大鼠随机分为正常组、肝郁组、脾虚组、肝郁脾虚组、肝郁干预组、脾虚干预组、肝郁脾虚干预组共7组,每组13只。3个证候模型组及各干预组大鼠分别采用慢性束缚、过度疲劳+饮食失节、慢性束缚+过度疲劳+饮食失节法造模,正常组不予处理;于造模第2周末,3个证候干预组大鼠按2.86 g/kg分别给予柴疏四君子汤灌胃,连续2周;各模型组和正常组给予等量蒸馏水。实验第14天和第28天,旷场实验法观察各组大鼠的行为特征,记录爬格数、直立次数、中央格停留时间、修饰时间及测算活动里程数。结果实验2周末时,与正常组相比,肝郁组和脾虚组大鼠爬格数、直立次数和里程数均显著升高(P0.05),肝郁脾虚组则均显著降低(P0.05);肝郁组和肝郁脾虚组中央格停留时间显著升高、修饰时间降低(P0.05),脾虚组变化与之相反(P0.05)。实验4周末时,与正常组相比,3个证候模型组大鼠爬格数和里程数均显著降低(P0.05),其中肝郁组和肝郁脾虚组还见直立次数和修饰时间显著降低(P0.05),中央格停留时间显著升高(P0.05),脾虚组的相关指标变化相反。中药干预2周后的变化:与相应模型组相比,肝郁干预组大鼠爬格数、里程数显著升高(P0.05),中央格停留时间显著降低(P0.05);脾虚干预组还见修饰时间显著升高(P0.05),而直立次数显著降低(P0.05);肝郁脾虚干预组各参数均显著升高(P0.05)。结论 3个证候模型大鼠在自主活动方面均存在不同程度的异常,其中肝郁证大鼠中枢的兴奋性由强转弱,脾虚证大鼠呈中枢神经系统反应失调,肝郁脾虚证大鼠呈现抑制渐进加重的趋势。柴疏四君子汤对3个证候模型的行为学指标均有一定的改善作用,但以对肝郁脾虚证的综合干预效应最优。

Behavioral changes in rats of liver-stagnation pattern,spleen-deficiency pattern and liver-stagnation with spleen-deficiency pattern and effects of Chaihu Sijunzi Decoction on different patterns

Objective To compare the behavioral changes of different rat models of liver-stagnation pattern,spleen-deficiency pattern,and liver-stagnation and spleen-deficiency pattern and to explore the effects of Chaihu Sijunzi Decoction (Bupleurum with Four Gentlemen Decoction)on those rat models. Methods Male Wistar rats were randomly assigned into the normal group (group 1 ),liver-stagnation group (group 2 ),spleen-deficiency group (group 3 ),liver-stagnation with spleen-deficiency group (group 4),liver-stagnation intervention group (group 5),spleen-deficiency intervention group (group 6)and liver-stagnation with spleen-deficiency intervention group (group 7)with thirteen rats in each group.The models were established by using chronic restraint method in group 2 and 5,by using excessive fatigue plus irregular diet method in group 3 and 6,and by using chronic restraint-excessive fatigue-irregular diet method in group 4 and 7 for 4 weeks.At the end of the 15 th day,rats in the group 5,6 and 7 were fed with Chaihu Sijunzi Decoction per 2.86 g·kg -1 for two weeks,whereas the rats in the normal group and model groups 2,3 and 4 were fed with equivalent saline for two weeks.On the 14th and 28 th day,open-fieldtest was used to observe the crossed squares (index C),number of rearing (index N),time remaining in the central zone (index S),decorating time (index D)and the total distance of movement (index T)of all rats.Results On the 14th day,as compared with group 1,index C,index N and index T increased significantly in group 2 and 3 (P <0.05)and decreased significantly in group 4 (P <0.05).Index S increased significantly in group 2 and 4 (P <0.05)while it decreased significantly in group 3 (P <0.05).Index D decreased significantly in group 2 and 4(P <0.05)and increased significantly in group 3 (P <0.05).On the 28 th day,compared with group 1,index C,index T decreased significantly in group 2,3 and 4 (P <0.05 ),while index N and index D decreased significantly and index S increased significantly in group 2 and 4 (P <0.05),which were totally opposite to group 3.Compared with group 2,index C and index T increased significantly while index S decreased significantly in group 5 (P <0.05).Compared with group 3,index C,index D,and index T increased significantly while index N decreased significantly in group 6(P <0.05).Compared with group 4,the crossed squares,number of rearing,decorating time,and index T increased significantly in group 7 (P <0.05).Conclusion The autonomic activities were at different degrees in rat models of liver-stagnation pattern,spleen-deficiency pattern,and liver-stagnation with spleen-deficiency pattern.There were decreased CNS excitement in the liver-stagnation pattern,disordered central nervous response in the spleen-deficiency pattern,and inhibited central excitatory activities in the liver-stagnation and spleen-deficiency pattern.Chaihu Sijunzi Decoction can improve the autonomic activities of those rat models,es-pecially of the liver-stagnation with spleen-deficiency model.