隔药灸命门穴对痛经模型大鼠子宫前列腺素表达的作用

目的观察隔药灸命门穴对痛经模型大鼠前列腺素(PG)表达的作用。方法将100只雌性SD大鼠,随机分为造模组(80只)和空白组(20只),用缩宫素制备大鼠痛经模型,造模成功后,将80只痛经模型大鼠随机分为模型组、药物组、针刺组、隔药灸组。药物组每天给予加味益母草膏(80 g/kg)灌胃,针刺组每天针刺命门穴,隔药灸组每天隔药(理中汤加减制成药饼)灸命门穴6壮。比较各组大鼠扭体反应的潜伏期和30 min内扭体次数,用免疫组织化学方法检测各组大鼠子宫内膜前列腺素F2α受体抗体(PGF2α-R)和前列腺素E2受体抗体(PGE2-R)表达水平。结果 1模型组有较多扭体次数,且有扭体潜伏期,而空白组无扭体,2组相比有统计学意义(P0.01)。与模型组相比,针刺组、药物组、隔药灸组的30 min内扭体反应次数均减少,且首次扭体潜伏期均明显延长(P0.01)。针刺组、药物组和隔药灸组扭体的潜伏期相比,差异无统计学意义(P0.05),但与药物组相比,针刺组及隔药灸组均能明显减少30 min内扭体反应次数(P0.01);且针刺组与隔药灸组比较,隔药灸组30 min内扭体次数最少(P0.01)。2与空白组比较,模型组、药物组、针刺组及隔药灸组PGF2α-R表达明显增多,PGE2-R表达明显减少(P0.01);与模型组比较,药物组、针刺组及隔药灸组PGF2α-R表达明显减少,PGE2-R表达明显增多(P0.01);与药物组比较,针刺组及隔药灸组PGF2α-R明显减少,PGE2-R表达明显增多(P0.01);与针刺组比较,隔药灸组PGF2α-R明显减少(P0.05),PGE2-R明显增多(P0.01)。结论隔药灸治疗原发性痛经与调节PGF2α-R及PGE2-R表达有关,即可下调痛经大鼠PGF2α-R表达,提高PGE2-R表达。

Effect of medicinal-cake-seperated moxibustion on expression of prosta-glandins in uterus of rat model with primary dysmenorrheal

Objective To observe the effect of medicinal-cake-separated moxibustion on the expression of prostaglandins (PG)in rats with primary dysmenorrheal (PD).Methods 20 of 100 female rats were included in blank group,and the rest 80 rats established PD model by injection of oxytocin and estrogen were randomized into model group,Chinese medicinal group (medicinal group)administered intragastri-cally with modified yimucao ointment once daily,acupuncture group treated with needling at point of Mingmen (GV4)once daily and medicinal-cake-separated moxibustion group(moxibustion group)with Lizhongtang at GV4 once daily.The latency duration and the times of writhing body (times)within 30 min were observed,and the serum levels of PGF2α-R and PGE2-R were detected by using immunohisto-chemical method.Results ①The times and latency duration in model group were more than those of blank group(P <0.01).Compared with model group,the times decreased significantly and the first la-tency duration prolonged in acupuncture group,Chinese medicinal group and moxibustion group(P <0. 01).There was no statistical difference in latency duration among the three treatment groups;however, compared with medicinal group,the times in 30 min decreased markedly in both acupuncture group and moxibustion group and the times in moxibustion group was less than those in acupuncture group(P <0. 01).②Compared with blank group,the expressions of PGF2α-R in the other four groups obviously in-creased (P <0.01),while the expressions of PGE2-R obviously decreased (P <0.01).Compared with model group,the expressions of PGF2α-R in three treatment groups were downregulated (P <0.01),while PGE2-R upregulated (P <0.01).Compared with medicinal group ,the expressions of PGF2α-R decreased significantly while PGE2-R increased in both acupuncture group and moxibustion group (P <0.01 ). Conclusion The mechanism of medicinal-cake-separated moxibustion on alleviating PD maybe takes into effect via downregulating the expression of PGF2α-R and upregulating expression of PGE2-R.