增液汤对慢传输型便秘模型小鼠结肠VIP及AQP3表达的影响

目的研究增液汤对慢传输型便秘模型小鼠结肠血管活性肠肽(VIP)及水通道蛋白3(AQP3)表达的影响,并探索VIP与AQP3表达之间是否存在相关性。方法 ICR小鼠60只,随机分成4组,即正常组,模型组,莫沙必利组,增液汤组。各组给予复方地芬诺酯灌胃建立小鼠慢传输型便秘模型,再给予相应的药物治疗。采用免疫组化法检测AQP3和VIP在各组结肠的分布和表达情况,测定平均光密度值。Pearson相关系数分析AQP3和VIP的表达相关性。结果与空白组相比,模型组小鼠结肠VIP及AQP3的平均光密度值均明显降低(P0.05);与模型组相比,莫沙比利组和增液汤组VIP及AQP3的值均显著增高(P0.05);增液汤组VIP及AQP3的表达与莫沙必利组无明显差异(P0.05)。各组VIP与AQP3的值呈正相关(P0.05)。结论增液汤可通过上调小鼠结肠VIP及AQP3的表达来治疗慢传输型便秘。小鼠结肠组织VIP与AQP3表达存在相关性,VIP与AQP3可能存在同一信号通路中。

Effects of Zengye Decoction on the expression of AQP3 and VIP in colon tissues of mice with slow transit constipation

Objective To investigate the effects of Zengye Tang (Fluid-increasing Decoction)on the ex-pression of Aquaporin-3 (AQP3)and Vasoactine Intrestinal Peptide (VIP)in colon tissues of mice with Slow Transit Constipation (STC)and to explore the correlation between AQP3 and VIP.Methods 60 ICR mice were randomly divided into 4 groups,namely control group,model group,mosapride group and Zengye Decoction group.Each group except control group was given diphenoxylate to establish the STC model.Control group was given saline.After the establishment of the model,Zengye Decoction group was given the Zengye Decoction while mosapride group was given mosapride.Control group and model group were given saline.The expression of AQP3 and VIP in colon tissues in each group was measured by using immunohistochemistry staining.Mean density (MD)was used to analyze the correlation between AQP3 and VIP.Results Mean density of AQP3 and VIP in colon tissues in model group were markedly lower than that in control group (P <0.05).Levels of AQP3 and VIP in mosapride group and Zengye Decoction group were significantly higher than that in model group (P <0.05 ).AQP3 and VIP in Zengye Decoction group were not statistically different from that in mosapride group (P >0.05).The lev-els of AQP3 and VIP in each group were positively correlated.Conclusions Zengye Decoction can in-crease the expression of AQP3 and VIP in colon tissues of STC mice,which may explains the mechanism of its efficacy.The expressions of AQP3 and VIP in colon tissues were correlated,probably due to a shared signal pathway.