17β-雌二醇和二噁英对子宫内膜间质细胞表达趋化因子IL-8及其受体CXCR1的调控作用

解析内外环境因素对子宫内膜间质细胞表达IL-8及其自分泌作用的调控。采用免疫组化法比较子宫内膜异位症患者异位灶和在位内膜CXCR1翻译水平表达;流式细胞术分析17β-雌二醇和二噁英单独或联合作用对子宫内膜间质细胞表面CXCR1表达的调控作用;ELISA法分析17β-雌二醇和二噁英单独或联合作用对子宫内膜间质细胞分泌IL-8的影响。结果显示CXCR1在子宫内膜异位症患者异位灶组织高表达。17β-雌二醇和二噁英单独作用均抑制子宫内膜间质细胞表面CX-CR1的表达以及IL-8的分泌。二者联合作用能够上调CXCR1的表达,上调幅度与雌二醇浓度呈正相关;但进一步抑制了IL-8的分泌。雌激素与二噁英对子宫内膜间质细胞复合作用抑制其IL-8的分泌及其自分泌作用;子宫内膜异位症患者腹腔液高水平IL-8并非由内外雌激素样物质直接作用于异位灶子宫内膜间质细胞所致。

Modulation of 17β-estradiol and/or TCDD on the expression of chemokine IL-8 and its receptor CXCR1 by endometrial stromal cells

To explore modulatory effect of the combined effect of estradiol and TCDD on IL-8 expression and auto-endocrine role in endometrial stromal cells(ESC).Immunohistochemistry was used to evaluate the protein translation of CXCR1 in eutopic endometrium and endometriotic tissue of patients with endometriosis.Treatment in vitro of the endometrial stromal cells by estradiol and/or TCDD was to evaluate the modulatory effect of estradiol and TCDD alone or in combination on the CXCR1 expression and IL-8 secretion by endometrial stromal cells.The CXCR1 translation in ESC was determined by flow cytometric assay,and the IL-8 secreting level in supernatant was detected by ELISA.It was found that CXCR1 was more translated by endometriotic tissue than eutopic endometrium.Both estradiol and TCDD inhibited the CXCR1 expression and IL8 secretion by ESC.The combination of estradiol and TCDD up-regulated the expression of CXCR1 but further inhibited the secretion of IL-8 by the ESC.The combination of estradiol and TCDD show an inhibition effect on IL-8 secretion and auto-endocrine role in ESC.Ectopic ESC exposed to estrogen-related milieu is not account for the increased IL-8 concentration in peritoneal fluid of patients with endometriosis.