大豆异黄酮对去势大鼠血脂及动脉内皮舒张功能的影响

目的探讨大豆异黄酮对去卵巢大鼠的血脂代谢及血管内皮依赖性舒张功能的影响.方法50只健康雌性SD大鼠随机分成五组,A组假手术组,B组去卵巢(OVX) 高脂饲料,C组为OVX 高脂饲料 20 mg/(kgbw·d)大豆异黄酮,D组为OVX 高脂饲料 60 mg/(kgbw·d)大豆异黄酮,E组为OVX 高脂饲料 180 mg/(kgbw·d)大豆异黄酮.实验第61天股动脉放血处死大鼠,取血清测血脂、取胸主动脉做内皮依赖性舒张功能检测.结果A、C、D、E组大鼠血清TC、TG、低密度脂蛋白胆固醇(LDL-C)含量都显著低于B组,C、D、E组血清高密度脂蛋白胆固醇(HDL-C)显著高于B组(P＜0.05);B组大鼠动脉对乙酰胆碱诱导的内皮依赖性舒张反应显著低于A、C、D、E组(P＜0.05).结论大豆异黄酮能抑制血清TG、TC、LDL-C的升高,同时升高HDL-C,保护动脉内皮的正常功能,从而有益于预防绝经妇女动脉粥样硬化的形成.

Effects of soybean isoflavone on the serum lipid metabolism and endothelial dilation function of artery in ovariectomized rats

Objective: To investigate the effect of soybean isoflavone on the serum lipid metabolism and the endothelium-dependent dilation function of artery in ovariectomized female rats. Methods: Fifty SD female rats, two months old, were randomly assigned to five groups: A (sham operated); B (ovariectomized and hyperlipid food); C (OVX and hyperlipid food and 20 mg/(kgbw · d) soybean isoflavone); D (OVX and hyperlipid and 60 mg/(kgbw · d) soybean isoflavone); E (OVX and hyperlipid and 180 mg/(kgbw · d) soybean isoflavone). Beginning from 7 days after surgery, the rats were treated intragastrically with soybean isoflavone or solvent once daily respectively for a period of 60 days. Serum TC, TG, HDL-cholesterol, LDL- cholesterol et al. Were measured. At the end of the experiments, endothelium -mediated dilation function of artery was also evaluated. Results: Group B had a significant increase in serum TC,TG,LDL- C. HDL- C contents were significant increased in C,D and E groups than that in group B; Moreover, soybean is oflavone could improve the endothelium- mediated dilation in ovariectomized female rats. Conclusions: Soybean isoflavone can not only decrease TG, TC and LDL - C, but also increase HDL - C. Soybean isoflavone can improve the endothelium - mediated dilation function of artery.