| X射线全身照射对小鼠胸腺细胞p53、p21、GADD45及MDM2蛋白表达的影响 |
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| 引用本文： | 鞠桂芝,傅海青,苏旭,付士波,刘树铮.X射线全身照射对小鼠胸腺细胞p53、p21、GADD45及MDM2蛋白表达的影响[J].吉林大学学报(医学版),1999,25(6):3. |
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| 作者姓名： | 鞠桂芝 傅海青 苏旭 付士波 刘树铮 |
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| 作者单位： | 吉林大学医学院卫生部放射生物实验室,长春,130021 |
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| 基金项目： | 国家自然科学基金资助项目（39770193） |
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| 摘 要： | 目的：观察电离辐射全身照射对小鼠胸腺细胞ｐ５３、ｐ２１、ＧＡＤＤ４５ 及ＭＤＭ２ 蛋白表达的影响。方法：采用间接免疫荧光标记，流式细胞术检测蛋白表达的变化。结果：２ Ｇｙ Ｘ射线照射后１～８ ｈ ｐ５３ 蛋白表达显著增高；照射后４～４８ ｈ ｐ２１ 蛋白表达显著增高；ＭＤＭ２ 蛋白在照后４ ｈ 及８ ｈ 显著增高；而ＧＡＤＤ４５ 蛋白表达在照后１～４８ ｈ 内无明显变化。结论：电离辐射可诱导胸腺细胞ｐ５３、ｐ２１ 及ＭＤＭ２ 蛋白表达增高。此结果将对辐射诱导胸腺细胞Ｇ１ 期阻滞的分子通路研究有重要意义。
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| 关 键 词： | 电离辐射 p53 p21 GADD45 MDM2 |
| 修稿时间： | 1999-01-08 |
Effect of whole body X-irradiation on the expression of p53,p21,GADD45 and MDM2 proteins in mouse thymocytes |
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| Ju Guizhi,Fu Haiqing,Su Xu,Fu Shibo,Liu Shuzheng.Effect of whole body X-irradiation on the expression of p53,p21,GADD45 and MDM2 proteins in mouse thymocytes[J].Journal of Jilin University: Med Ed,1999,25(6):3. |
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| Authors: | Ju Guizhi Fu Haiqing Su Xu Fu Shibo Liu Shuzheng |
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| Institution: | MH Radiobiology Research Unit |
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| Abstract: | Objective:To investigate the effect of ionizing radiation on the expression of p53,p21,GADD45 and MDM2 proteins of thymocytes in mice.Methods:Flow cytometry (FCM) was used to analyze protein expression of thymocytes for their immunofluorescence stained with monoclonal antibodies.Results:It was demonstrated that after 2 Gy whole body irradiation with X rays,p53 protein expressions of mouse thymocytes were significantly increased at 1 h to 8 h;p21 at 4 h to 48 h; and MDM2 at 4 h and 8 h.However,no significant change was found in GADD45 protein expression following irradiation.Conclusion:The protein expression of p53,p21 and GADD45 of thymocytes could be induced by ionizing radiation,which might be involved in the molecular pathway of G 1 arrest after exposure. |
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| Keywords: | p53 p21 GADD45 MDM2 |
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