柴胡对大鼠实验性肝纤维化的预防作用

目的研究中药柴胡对肝纤维化的预防作用。方法采用四氯化碳(CCL4)诱发大鼠肝损伤,辅以高脂、低蛋白饲料和一定浓度乙醇饮料,复制肝纤维化模型,柴胡预防给药,观察其对大鼠血清及肝组织生化指标的影响。结果柴胡预防给药可显著降低四氯化碳所致慢性肝损伤大鼠血清ALT、AST和肝组织MDA、HyP,提高肝组织SOD活性。病理组织学观察显示,柴胡可使肝组织变性、坏死程度减轻。结论柴胡能预防四氯化碳致大鼠肝纤维化的发生与发展。

Prophylactic effect of bupleurum polyclonum on hepatic fibrosis in rats

Objective To study the prophylactic effect of traditional Chinese medicine bupleurum polyclonum(BP) on hepatic fibrosis in rats.Methods Experimental model of hepatic fibrosis was induced by carbon tetrachloride(CCL_4) and supplementary high-lipid and low-protein diet and alcohol-containing beverage.Serum and liver biochemical markers and collagen content were tested to evaluate the prophylactic effect of bupleurum polyclonum on hepatic fibrosis.Results Pretreatment with BP could decrease the activities of ALT and AST in serum,reduce the MDA content,increase the SOD activity,prevente the increase of HyP in liver tissue in CCL_4-induced chronic liver injury model rats.Histomorphological studies showed that the degeneration and necrosis were decreased in prophylactic group.Conclusion BP could prevent the genesis and development of hepatic fibrosis induced by carbon tetrachloride in rats.