| 紫外分光光度法测定柴胡中多糖含量 |
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| 引用本文： | 关皎,朱鹤云,刘洋,王宏玉,郝乘仪,冯波.紫外分光光度法测定柴胡中多糖含量[J].吉林医药学院学报,2016(6). |
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| 作者姓名： | 关皎 朱鹤云 刘洋 王宏玉 郝乘仪 冯波 |
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| 作者单位： | 吉林医药学院药学院，吉林吉林 32013 |
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| 基金项目： | 吉林省卫生计生委科研课题(2013Z008)；吉林省中医药管理局科研课题(2015Z726)． |
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| 摘 要： | 目的：采用紫外分光光度法测定柴胡中多糖含量。方法采用苯酚-硫酸显色法，以葡萄糖为对照品，490 nm为检测波长。结果测得柴胡中多糖的平均含量为15．8％，平均回收率为98．9％。结论该方法简便、快速且提取率高，可用于柴胡中多糖的含量测定。
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| 关 键 词： | 紫外分光光度法 柴胡 多糖 |
Determination of polysaccharide in Bupleuri Radix by UV |
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| Abstract: | Objective To develop an UV method for the determination of polysaccharide in Bupleuri Radix.Meth-ods The phenol-sulfuric acid was used as chromogenic agent , and glucose was used as the reference standard .The detection wavelength was set at 490 nm.Results The average content of polysaccharide was 15.8%,and the aver-age recovery rate was 98.9%.Conclusion The method is simple ,rapid and has high extraction rate ,which can be used for the determination of polysaccharide in Bupleuri Radix. |
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| Keywords: | UV polysaccharide Bupleuri Radix |
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