| HPLC法测定四子补益膏剂中特女贞苷的含量 |
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| 引用本文： | 刘东梅,张伟,柳絮,雷钧涛.HPLC法测定四子补益膏剂中特女贞苷的含量[J].吉林医药学院学报,2016(6):421-422. |
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| 作者姓名： | 刘东梅 张伟 柳絮 雷钧涛 |
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| 作者单位： | 1. 吉林省吉林中西医结合医院,吉林吉林,132012;2. 吉林医药学院,吉林吉林,132013 |
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| 基金项目： | 吉林省中医药管理局(2014-Z12)． |
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| 摘 要： | 目的：建立四子补益膏剂中特女贞苷的含量测定方法。方法采用高效液相色谱（ HPLC）法测定四子补益膏剂中特女贞苷的含量，色谱柱采用Luna C18柱（4．6 mm ×250 mm，5μm）；乙腈-0．01 mol／L磷酸二氢钾（23∶77）为流动相，检测波长224 nm，柱温25℃；流速为0．7 mL／min。结果测得特女贞苷在0．05～1．6 g／L浓度范围内与特女贞苷峰面积之间具有良好的线性关系（R＝0．9999），平均回收率为97．1％，RSD为0．75％（ n＝6）。结论该方法准确可靠、重复性好可用于测定四子补益膏剂中特女贞苷的含量。
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| 关 键 词： | 特女贞苷 四子补益膏剂 含量测定 高效液相色谱法 |
Determination of specnuezhenide in Sizi Buyi Gao by HPLC |
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| Abstract: | Objective To establish a method for the determination of specnuezhenide in Sizi Buyi Gao .Methods Luna C18 column was used .The mobile phase was consisted of acetonitrile-0.01 mol/L and potassium dihydrogen phosphate (23∶77 ) .The UV detector was set at 224 nm.The column temperature was 25 ℃.The flow rate was 0.7 mL/min.Results The linear response range was 0.05~1.6 mg/mL(R=0.999 9).The average recovery of specnuezhenide was 97.1%,and RSD was 0.75%.Conclusion The method was accurate,simple and repeatable, which can be applied in quantitative determination of specnuezhenide in Sizi Buyi Gao . |
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| Keywords: | specnuezhenide Sizi Buyi Gao determination HPLC |
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