槲皮素对胃肠运动的影响及其机制

目的:研究槲皮素对小鼠离体结肠收缩活动以及胃排空、肠推进的影响, 探讨槲皮素作用于胃肠道的药理学机制.方法:将昆明小鼠随机分为对照组、槲皮素组(低、中、高剂量)、阿托品组、新斯的明组、阿托品加槲皮素组以及新斯的明加槲皮素组. 采用紫外分光光度计测量葡聚糖蓝-2000在小鼠胃内色素残留率及小肠各肠段色素残留率, 观察槲皮素对小鼠胃排空及小肠推进功能的影响. 采用TSD125C型张力传感器及MP150放大器测量结肠平滑肌收缩与舒张的幅度.结果:槲皮素能够抑制正常小鼠胃排空及小肠推进, 高剂量槲皮素组胃残留量与对照组比较差异显著(27.10%±3.25% vs 12.79%±3.30%, P <0.01), 对照组葡聚糖蓝-2000主要集中在小肠后段, 槲皮素组葡聚糖蓝-2000主要集中在小肠前段. 槲皮素可加强阿托品对小肠推进抑制作用, 但对阿托品抑制胃排空的作用没有影响(胃残留量:28.35%±17.36% vs26.64%±14.91%, P >0.05); 他能够拮抗新斯的明引起的胃排空(胃残留量:10.28%±4.84%vs 1.86%±1.01%, P <0.01)及推进小肠亢进. 槲皮素对小鼠离体结肠具有舒张作用, 且随着槲皮素的浓度升高, 槲皮素(0.25-50 μmol/L)舒张作用增强. 槲皮素的舒张作用与NO途径及PKC途径无关.结论:槲皮素具有舒张胃肠平滑肌的作用.

Effect of quercetin on gastrointestinal motility and its mechanism

AIM: To investigate the effect of quercetin on the contractility of colon muscle in vitro,gastric emptying and intestinal propulsionfunction of mouse in vivo, then to discuss thepharmacological mechanism of quercetin on the gastrointestinal movement.METHODS: Kun-ming mice were randomly dividedinto normal control group, quercetin (low dose, middle dose, high dose) groups, atropinegroup, neostigmine group, atropine and quercetingroup, and neostigmine and quercetin group.Dextran blue -2000 was used as a marker to investigatethe effects of the quercetin on gastric emptying and motility of small intestine in mice.Colon muscle strips from the distal segment of mice colon, which were taken at 1 cm from theanus approximately, were used to take contractile tension recording.RESULTS: There is no significant difference between the low-dose group and the controlgroup, but gastric emptying was significantlyinhibited in the high-dose group compared with the control group (gastric residue: 27.10% ± 3.25%vs 12.79% ± 3.30%, P < 0.01). The quercetin inhibitedthe activity of the small intestine, the controlgroup of Dextran blue-2000 mainly was concentrated in the posterior segment of small intestine meanwhile the quercetin group mainly in thepreceding. Quercetin strengthened the inhibitory effect of atropine on small intestine, but therole of its inhibition did not affect gastric emptying(gastric residue: 28.35% ± 17.36% vs 26.64% ±14.91%, P>0.05). It antagonized gastric emptying(gastric residue: 10.28% ± 4.84% vs 1.86% ±1.01%, P<0.01) and inhibited hyperfunction of the small intestine induced by neostigmine. Invitro quercetin relaxed the distal colon section of mice in a dose-dependent (0.25-50 μmol/L)manner, the effect of quercetin was not via No pathway or PKC pathway.CONCLUSION: These results indicate that quercetincan