大鼠失神经支配后外源性NGF对骨质疏松的影响

目的 观察失神经支配后外源性NGF(神经生长因子)对大鼠体重、血钙及骨结构的影响，进而说明NGF在神经源性骨质疏松康复中的意义。方法 雄性SD大鼠随机分为对照组和失神经组，失神经组大鼠切断股骨神经造成失神经支配模型，然后分为失神经支配组和失神经支配注射NGF组，30d后称重、取血、处死动物取股骨进行骨组织计量学检查。结果 失神经支配大鼠注射NGF后，体重、骨小梁量与未注射组相比具有明显增加，提示局部予以NGF治疗可明显减轻失神经支配后大鼠骨质疏松的程度，并可增加因失神经导致的体重减轻，而各组的血钙磷浓度无明显改变。结论 说明神经性骨质疏松的发生并非源自钙磷流失，而是由骨小梁的结构改变所致，同时外源性NGF在神经性瘫痪后骨质疏松的康复中具有积极意义。

Effect of nerve growth factor on osteoporosis in denervated rats

Objective To study the effect of nerve growth factor(NGF) on body weight, blood calcium level and bone histomorphometry in denervated rats, thereby providing a therapeutic method for rewvery of neuropathic paralysis. Methods SD male rats were divided into control group and denervated group, The denervated group underwent neurotomy on bilateral sciatic nerves and then randomly divided into denervatied control group and NGF injection group. The body weight and blood calcium level were measured and the bone morphometry was done by dual energy absorptiometry after the NGF injection group was injected with NGF for 30 days. Results At the end of the experiments, the weight and the microstructure of bone trabeculae in denervatied control group were changed significantly, whereas those in the NGF injection group were significatly increased. Conclusions The neuropathic osteoporosis results from changes in microstructure of bone, not from decrease in calcium. NGF therapy can prevent the osteoporosis, indicating that NGF therapy is very important for rewvery of netropathic paralysis.