| 白血病细胞系中B-Raf与Raf-1的表达及活性 |
| |
| 作者姓名： | Zhang J Liu Y Liu S Zheng D |
| |
| 作者单位： | [1]中国医学科学院中国协和医科大学基础医学研究所医学分子生 [2]中国医学科学院中国协和医科大学基础医学研究所医 |
| |
| 摘 要： | 目的：研究白血病细胞及正常人外周血淋巴细胞中的Ｂ－Ｒａｆ和Ｒａｆ－１表达水平及激酶活性。方法：利用Ｗｅｓｔｅｒｎ印迹法检测Ｂ－Ｒａｆ和Ｒａｆ－１表达水平,利用免疫沉淀及Ｗｅｓｔｅｒｎ印迹法检测Ｒａｆ－１及Ｂ－Ｒａｆ的激酶活性。结果：Ｒａｆ－１在白血病细胞及正常人外周血淋巴细胞中均有表达,表达水平基本相似,其激酶活性较低;Ｂ－Ｒａｆ仅在白血病细胞中表达,且在Ｊｕｒｋａｔ和Ｋ５６２细胞中的表达水平较高,其激酶活性也较高。结论：在白血病细胞中,Ｂ－Ｒａｆ的高表达及活化可能是白血病发病的机制之一。
|
| 关 键 词： | Raf-1 B-Raf 激酶活性 白血病细胞 |
Expression and kinase activity of B-Raf and Raf-1 in leukemia cell lines |
| |
| Zhang J,Liu Y,Liu S,Zheng D.Expression and kinase activity of B-Raf and Raf-1 in leukemia cell lines[J].Acta Academiae Medicinae Sinicae,2000,22(6):533-535. |
| |
| Authors: | Zhang J Liu Y Liu S Zheng D |
| |
| Institution: | National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China. |
| |
| Abstract: | OBJECTIVE: To study expression and kinase activity of B-Raf and Raf-1 kinases in several leukemia cells and normal peripheral blood lymphocytes. METHODS: Western blot was carried out to detect expression of B-Raf and Raf-1 in peripheral blood lymphocytes and leukemia cells. Kinase activities of Raf-1 and B-Raf were analyzed by immunoprecipitation and Western blot. RESULTS: The expression levels of Raf-1 were similar in tested leukemia cells and normal peripheral blood lymphocytes, and Raf-1 protein was all minimally tyrosine-phosphorylated. B-Raf expressed in all tested leukemia cells, but not in normal peripheral blood lymphocytes, and B-Raf was highly activated in Jurkat and K562 leukemia cells. CONCLUSION: The abnormal expression and kinase activity of B-Raf in leukemia cells might be related to leukemia etiologically. |
| |
| Keywords: | |
| 本文献已被 维普 万方数据 PubMed 等数据库收录！ |
