锯叶棕提取物对人脑胶质瘤细胞凋亡和PI3K蛋白表达影响的研究

目的：通过锯叶棕提取物对人脑胶质瘤细胞的干预，观察人脑胶质瘤细胞中细胞凋亡和PI3K蛋白表达的变化。方法体外培养U251细胞，应用锯叶棕提取物进行预处理，应用TUNEL法检测细胞凋亡，应用Western Blot法检测PI3K蛋白表达水平的变化。结果TUNEL法染色检测锯叶棕提取物对人脑胶质瘤细胞凋亡的影响，24 h与48 h均可见TUNEL阳性反应细胞即凋亡细胞，胞体缩小，形态不规则，胞核固缩浓染，呈棕黄色或棕褐色；1.5μL/mL组48 h与24 h比较，凋亡数量显著增多（P＜0.01）；对照组24 h与48 h偶见凋亡细胞，2.0μL/mL组与1.5μL/mL组比较，凋亡率增大（P＜0.01）。用Western blot法检测锯叶棕提取物对人脑胶质瘤细胞PI3K蛋白表达的影响，锯叶棕提取物预处理U251细胞与对照组比较， PI3K蛋白表达水平显著降低（P＜0.01）。结论锯叶棕提取物能诱导人脑胶质瘤细胞凋亡；锯叶棕提取物诱导人脑胶质瘤细胞凋亡的机制可能是通过阻断PI3K/Akt信号转导通路。

The Study of Saw Palmetto Extract on the Effect of Glioma Cell Apoptosis and Expression of PI3K Protein

Objective To observe the effect of saw palmetto extract on glioma cell apoptosis and the influence of PI3K in glioma cells through the intervention of saw palmetto on glioma cell. Methods U251 cells are cultured in vitro, pre-treatment with saw palmetto extract, to detect the cell apoptosis by TUNEL method, to detect the changes of the expression of PI3K by Western blot. Results 1.TUNEL method staining to detect the effect of saw palmetto extract on glioma cell apoptosis. （1）Both 24 h and 48 h time point see TUNEL positive cells （apoptotic cells）, cell body narrow, irregular, nucleus condensation stain, showed brown yellow or brown, 48 h and 24 h compare, the number of apoptotic cells significantly increased in 1.5μL/mL group （P〈0.01）;（2）24 h and 48 h time point occasionally see apoptotic cells in the control group, the same time 1.5μL/mL and 2.0μL/mL group, number of apoptotic cells significantly increased （P〈0.01）. 2.The effect of saw palmetto extract on glioma cell of expression of PI3K protein by Wersten Blot method. Saw palmetto preconditioning, compared with control group, expression of PI3K protein levels were significantly decreased （P〈0.01）. Conclusion Saw palmetto extract induced apoptosis in glioma cells. Saw Palmetto by blocking PI3K/Akt Signal Transduction Pathways can induce the glioma cell apoptosis.