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Oxytocin in the central nervous system and sexual behaviour in male rats
Authors:Andy M Hughes  Barry J Everitt  Stafford L Lightman  Kathryn Todd
Institution:1. Departments of Anatomy, Cambridge University, Cambridge ,U.K.;2. Departments of Medicine, Charing Cross and Westminster Medical School, Westminster Hospital, London ,U.K.;1. University of Oulu, Oulu, Finland;2. University of Turku, Turku, Finland;3. Institute of Biology and Soil Science, Russian Academy of Sciences, Vladivostok, Russia;4. U.S. Geological Survey, Marrowstone Marine Field Station, Nordland, WA, USA;5. Faculty of Biology, University of Gdańsk, Gdańsk, Poland;1. Department of Medicine, Division of Medical Oncology, University of Washington, Seattle, Washington;3. Department of Laboratory Medicine, University of Washington, Seattle, Washington;5. Division of Gastroenterology, University of Washington, Seattle, Washington;2. Fred Hutchinson Cancer Research Center, Clinical Research Division, Seattle, Washington;6. Division of Gynecologic Oncology, The Ohio State University, Columbus, Ohio;8. Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University, Columbus, Ohio;9. Division of Human Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio;4. Division of Medical Oncology, Department of Medicine, Stanford University, Stanford, California;7. Department of Surgery, The Ohio State University College of Medicine, Columbus, Ohio;1. Department of Biological Sciences, Faculty of Science and Engineering, University of Waikato, Hamilton, New Zealand;2. University of Auckland, Waikato Clinical School, Hamilton, 3240 New Zealand;3. Department of Food Science and Nutrition, University of Minnesota, St Paul, MN, USA;1. Department of Behavioral and Molecular Neurobiology, University of Regensburg, Regensburg, Germany;2. Technische Universität Dresden, University Hospital, Department of Internal Medicine III, Dresden, Germany;1. Department of Neuroscience, University of Turin, 10126 Turin, Italy;2. Department of Life Sciences and Systems Biology, University of Turin, 10123 Turin, Italy;3. Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy;4. NICO – Neuroscience Institute Cavalieri Ottolenghi, 10143 Orbassano, Italy
Abstract:Concentrations of oxytocin (OT) and vasopressin (AVP) were measured in cerebrospinal fluid (CSF) obtained from the cisterna magna of freely moving rats. Basal levels of OT and AVP were approximately 9 fmol/ml in both male and female. In the male rats this increased to approximately 18 fmol/ml 5 min after ejaculation, and 27 fmol/ml 20 min after ejaculation. No increase from basal levels occurred when males were placed with unreceptive females, or alone in the test environment. AVP levels were unchanged in any condition. Preliminary investigations indicate that discrete electrolytic lesions to the lateral and posterior parvocellular hypothalamic paraventricular nucleus (PVN) abolished this ejaculation-associated increase in CSF OT, prolonged mount and intromission latencies and reduced the absolute postejaculatory interval (PEI). We conclude that intracerebrally projecting OT systems may be activated during coitus and may contribute to the mechanisms underlying postejaculatory refractoriness.
Keywords:Cerebrospinal fluid  Oxytocin  Paraventricular nucleus  Postejaculatory interval  Sexual behavior
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