生长激素受体mRNA在肝细胞癌和癌旁肝硬化组织中的表达

背景与目的:正常肝组织富含生长激素受体mRNA,但在肝细胞癌、癌旁肝硬化组织中生长激素受体mRNA的表达情况不详,因此,本文旨在探讨肝癌组织、癌旁肝硬化组织中生长激素受体mRNA的表达情况。方法:采用逆转录-聚合酶链反应方法检测37例肝癌患者肝癌组织、癌旁肝硬化组织中生长激素受体mRNA的表达。结果:肝癌组织生长激素受体mRNA表达率(30/37,81.0%)较癌旁肝硬化组织的表达率(32/32,100%)低(P<0.05),未分化肝癌的表达率(1/4,20.0%)更低(P<0.5);癌旁肝硬化组织的生长激素受体mRNA的表达量犤积分光密度比值(riOD)为(30.77±8.24)%,n=32犦较正常对照肝组织的表达量犤riOD为(44.93±6.25)%,n=5犦少(P<0.05),重度肝硬化的表达量犤riOD为(21.90±4.72)%,n=8犦更少(P<0.05)。结论:生长激素受体mRNA的表达下调与肝癌的分化程度、癌旁肝硬化的严重程度有关。

Expression of growth hormone receptor mRNA in hepatocellular carcinoma and matched para-cancer cirrhotic liver tissue

BACKGROUND & OBJECTIVE: The growth hormone receptor mRNA is abundant in normal liver tissue, but its expression was not studied in detail in the tissue of liver cancer or para-cancer cirrhotic liver. This study was designed to investigate the expression of growth hormone receptor mRNA in hepatocellular carcinoma and para-cancer cirrhotic liver tissue. METHODS: The expression of growth hormone receptor mRNA was detected in 37 specimens of hepatocellular carcinoma and their para-cancer liver tissue by RT-PCR. RESULTS: The expression rate of growth hormone receptor mRNA in hepatocellular cancerous tissue (30/37, 81%) was significantly lower than that in cirrhotic liver tissue (32/32, 100%) (P < 0.05). The expression rate in undifferentiated hepatocellular carcinoma (1/4, 20%) decreased further (P < 0.05). The expression of growth hormone receptor mRNA in cirrhotic liver tissue ratio of integrated Optical Density (riOD) 30.77% +/- 8.24%, n = 32] was significantly lower than that in normal control liver tissue (riOD 44.93% +/- 6.25%, n = 5) (P < 0.05). The expression in severe cirrhotic liver tissue (riOD 21.90% +/- 4.72%, n = 8) decreased further (P < 0.05). CONCLUSIONS: The down-regulated expression of growth hormone receptor mRNA was related to the pathological stage of hepatocellular cancer and the stage of liver cirrhosis.