顶空固相微萃取-气相色谱-质谱联用法分析松花粉挥发性成分

采用顶空固相微萃取-气相色谱-质谱联用法(HS-SPME-GC-MS)分离,鉴定了松花粉中的挥发性成分。实验中筛选了固相微萃取纤维,优化了SPME的操作条件。样品在70℃下,用100μm聚丙烯酸酯(PA)固相微萃取纤维对松花粉样品顶空吸附30 min,于250℃脱附10 min然后采用GC-MS对解析物进行分离鉴定。采用HS-SPME-GC-MS测得85个峰,鉴定出70种组分,主要为脂肪类化合物。结果表明:HS-SPME可用于松花粉中挥发性成分的快速分析。

Headspace Solid-phase Microextraction-Gas Chromatography-Mass Spectrometry for Analysis of Volatile Components from Pollen Pini

Headspace solid-phase microextraction HS-SPME) technique was employed to extract the volatile compounds from Pollen Pini. The volatile components were isolated and identified successfully by gas chromatography-mass spectrometry (GC-MS). The volatiles were collected using several SPME parameters (including extraction time and temperature). The best response was obtained using a PA fiber by when the extraction temperature was 70 degrees C, extraction time was 30 minutes and desorption time was 10 time. Analysis was performed by GC-MS. Seventy components were identified by HS-SPME. The main components were fatty compounds. The results showed that the HS-SPME technique could be used to extract the volatile components from HS-SPME-GC-MS.