自发性癫痫大鼠海马区谷氨酸转运体GLAST表达下调

[目的]通过对照实验探讨自发性癫痫大鼠（spontaneously epilepsy rats,SER）与正常Wistar大鼠海马区胶质细胞型谷氨酸转运体GLAST蛋白的表达情况,以期发现其与遗传性癫痫发病的关系。[方法]利用聚合酶链反应（PCR）扩增SER的特异性基因片段,筛选出SER;利用免疫荧光定位GLAST蛋白的表达,并应用免疫组织化学技术,检测GLAST蛋白在SER及正常Wistar大鼠海马内的表达情况。[结果]GLAST蛋白在SER海马的齿状回（DG）表达与正常Wistar大鼠相比明显减少（GLAST的表达为Wistar：52.67±11.5,SER：39.39±9.48,P〈0.05）,在海马CA1和CA3区表达差异无显著性意义（CA1区为Wistar：48.56±10.6,SER：45.84±7.20;CA3区为Wistar：43.29±10.6,SER：36.73±6.50,P〉0.05）。[结论]SER自发性癫痫可能与海马DG区GLAST蛋白表达下降有关;GLAST表达下降可能是由SER基因位点的突变引起的。

Expression of glutamate transporters GLAST protein in hippocampus of spontaneously epileptic rats

[Objective] To investigate the expression of glutamate transporters GLAST protein in hippocampus of spontaneously epileptic rats(SER) and further explore the role of GLAST in the occurrence of epilepsy. [Methods] The SER was resieued by DNA extraction and PCR.The expression location of GLAST protein were detected by immunohischemistry and immunofluorensence respectively.[Results] The GLAST protein expmessien was lower in dentate gyrus(DG) of hippocampus in SER than that in cantral rats.There was not signifi...