神经生长因子对兔脊髓前角运动神经元保护作用的定量研究

钳夹损伤兔右坐骨神经，于损伤处注射蛇毒ＮＧＦ４００Ｂｕ／ｋｇ／日，损伤术后１，３，７天和２，３，４，６，８周动态观察脊髓腰段伤侧第ＩＸ板层外侧的大型运动神经元的ＡＣＰａｓｅ活性改变并应用图像分析仪对其进行定量研究。结果表明术后１，３天实验组和对照组ＡＣＰａｓｅ活性均增加；术后３周实验组ＡＣＰａｓｅ活性达高峰，溶酶体数目及溶酶体、高尔基复合体上的电子密度也明显增加，对照组酶活性不如实验组高，组间差异显著（Ｐ＜００１）。术后６周实验组ＡＣＰａｓｅ活性恢复至正常水平，而对照组ＡＣＰａｓｅ活性才开始回降。本研究显示蛇毒ＮＧＦ对坐骨神经损伤后脊髓前角运动神经元ＡＣＰａｓｅ活性恢复有促进作用，从而对运动神经元可起一定的保护和促进恢复的作用

Quantitative study on the protective effect of nerve growth factor (NGF) on motor neurons of anterior horn in rabbits

The right sciatic nerves of rabbits in experimental and control groups were crushed. The animals of the experimental group were locally injected with snake venom NGF (400 Bu/kg/day), 1,3,7 days and 2,3,4,6,8 weeks after nerve crush, the changes of acid phosphatase (ACPase )activity in motor neurons of the lumbar spinal cord were observed under LM and EM. The changes of ACPase on LM sections were detected with an image analysor and the data were analyzed statistically. The ACPase activity in the experimental and control group was increased during 1,3 days after nerve injury. But the ACPase activity of the experimental group was increased to the highest values 3 weeks after nerve injury, and there was significant difference between experimental group and control group (P<0.01), and the number of lysosomes increased considerably. Meanwhile, the electron density in lysosomes and Golgi apparatus was increased remarkably. As compared with ACPase activity of control group, the enzyme activity of experimental group was lower. The ACPase activity of the experimental group declined to the baseline 6 weeks after nerve injury (P<0.01). Otherwise, the ACPase activity of the control group just began to subside gradually. The results indicate that snake venom NGF could promote the recovery of the enzyme activity of ACPase of the motor neuron in the anterior horn of the spinal cord following sciatic nerve crushed. Therefore. snake venom NGF may play an important role in protecting the motor neuron and accelerating the recovery of the injured motor neuron.