MMP-2和MMP-13与乳腺癌腋窝淋巴结转移的相关性

目的本研究按照病理诊断中有无脉管癌栓分组，通过检测基质金属蛋白酶（MMPs）重要家族成员MMP、2，-9，-13，-14的表达；探讨乳腺癌转移中MMPs作用及其与淋巴结转移的关系。方法采用荧光定量RT—PCR检测30例乳腺癌组织MMP-2，一9，-13，一14mRNA表达情况；用免疫组化S-P方法检测乳腺癌组织中雌激素受体（ER）、孕激素受体（PR）、癌基因（HER2）、抑癌基因（P53）、核增殖抗体（Ki一67）的表达；采用t检验等方法处理实验数据。结果乳腺癌脉管癌栓阳性组淋巴结转移个数显著多于阴性组；脉管癌栓阴性组中MMP一2和MMP一13的表达较阳性组显著增高且差异有统计学意义（P〈0．05）。结论乳腺癌脉管癌栓阳性组有更明显的淋巴结转移趋势。肿瘤组织中MMP．2和MMP．13对肿瘤细胞的内渗起着负调控的作用。

The correlation between MMP-2, MMP-13 and the axillary lymph node metastasis in breast cancer

Objective To study the function of the important members of MMPs in breast cancer and their correlation with lymph nodes metastasis through making groups according to vascular thrombosis status showed by pathological diagnosis and detecting the expression of MMP-2,-9,-13,-14 in order.Methods Fluorescent quantitative RT-PCR was used to detect mRNA expression of MMP-2,-9,-13,-14 in 30 cases of breast cancer patients in both groups (negative and positive vascular thrombosis groups).Streptavidin-peroxidase methods (S-P) immunohistochemical method was employed to detect the expression of ER,PR,HER2,P53 and Ki-67 in breast cancer tissues.The data were analyzed by t test etc.Results Lymph nodes metastasis was significantly more in vascular thrombosis positive group than in negative group(P ＜ 0.05 ).MMP-2 and -13 were overexpressed in vascular thrombosis negative group (P ＜ 0.05).Conclusions Breast cancer patients with positive vascular thrombosis have a more apparent trend of lymph nodes metastasis.MMP-2 and MMP-13 mRNA play a negative regulatory role in intravasation of tumor cells by producing substances that may inhibit tumor angiogenesis and intravasation.