普罗布考对阿霉素肾病大鼠抗氧化作用的研究

目的：观察普罗布考对阿霉素肾病大鼠的抗氧化作用,并探讨其肾脏保护作用的机制。方法：雄性SD大鼠40只,随机分为正常对照组（A组）、造模后未治疗组（B组）、造模后普罗布考正常剂量治疗组（C组）、造模后普罗布考高剂量治疗组（D组）。尾静脉一次性注射阿霉素6mg/kg制备阿霉素肾病模型。1周后开始普罗布考药物干预,持续4周。检测大鼠24h尿蛋白定量,血清和肾组织中超氧化物歧化酶（SOD）、丙二醛（MDA）水平,同时观察肾组织病理学改变。结果：造模后C组、D组24h尿蛋白排泄量明显低于B组（P〈0.05）,C、D组血清和肾组织中SOD明显上升、MDA明显下降（P〈0.05）,肾组织病理损伤减轻。结论：普罗布考能提高阿霉素肾病大鼠血和肾组织中SOD活性,降低MDA水平,降低蛋白尿,减轻肾组织的病理改变,具有肾保护作用。

Study on Anti-oxidation Effect of Probucol in Rat With Adriamycin Induced Nephropathy

Objective：To observe the anti-oxidation effect of Probucol in rat with adriamycin induced nephropathy,and explore its protective mechanims.Methods：Forty male SD rats were randomly divided into 4 groups：normal group（group A）,model group（group B）,normal dose treatment group（group C） and high dose treatment group（group D）.The models of adriamycin-induced nephropathy were established by injection of 6mg/kg adriamycin into the tail vein.After one week,medical interventions were initiated and lasted for 4 weeks.Level of 24h urinary protein was measured,contents of SOD and MDA were measured,and renal pathologic changes were examined.Results：Compared with that of model group,24 h urinary protein was significantly decreased in group C and group D（P0.05）.The SOD activity was significantly increased and MDA was significantly decreased in group C and group D（P0.05）,renal pathologic changes were improved.Conclusions：Probucol has an protective effect which can improve SOD activity,decrease MDA content,decrease the urinary protein and improve the renal pathologic changes.