雄黄对T淋巴细胞白血病细胞系CEM的促凋亡作用

目的 观察雄黄对人类T淋巴细胞白血病细胞凋亡的影响。方法 人类T淋巴细胞白血病细胞系CEM体外培养，采用MTT法测定对细胞生长的抑制率，流式细胞仪分析细胞周期，并检测凋亡相关蛋白Apo2．7和Fas的表达。结果 雄黄体外对CEM细胞的生长有抑制作用，流式细胞仪检测可见亚二倍体的凋亡细胞和凋亡相关蛋白Apo2．7表达的升高，对细胞周期的影响表现为G2／M期的阻滞，而对Fas蛋白的表达没有影响。结论 雄黄有诱导CEM细胞凋亡的作用。

Study on Realgar inducing apoptosis in T lymphocytic cell line CEM

Objective To study the Realgar induced T lymphocytic leukemia cell line CEM apoptosis in vitro. Methods CEM cells were incubated with Realgar. Cell proliferation inhibition was determined by MTT . Cell cycle, apoptosis, Apo2.7 and Fas were measured by cytometer. Results Realgar inhibited the proliferation of CEM cell line. The cells treated with Realgar showed a Sub G 0/G 1 apoptotic peak in DNA distribution histogram, increment of Apo2.7 protein expression, and arrested cells in G 2/M phase, but ineffectiveness on Fas expression. Conclusion The Realgar can induce CEM cell apoptosis in vitro.