弓形虫p30-Rop2复合基因大肠杆菌/分枝杆菌穿梭质粒的构建

目的：构建弓形虫p30—Rop2复合基因大肠杆菌／分枝杆菌穿梭表达质粒。方法：利用PCR技术从已构建的pET30-p30-Rop2质粒扩增p30-Rop2复合基因片段，亚克隆于大肠杆菌／分枝杆菌穿梭质粒pSTM3，构建重组质粒pSTM3-p30-Rop2，并进行双酶切鉴定和测序分析。结果：成功构建pSTM3-p30-Rop2重组穿梭表达载体，测序结果发现有一个碱基发生突变，但没有改变开放阅读框。结论：pSTM3-p30—Rop2穿梭质粒的成功构建为弓形虫分枝杆菌疫苗的研制提供了前提条件。

Construct and Identification of Recombinant M.S-p30-Rop2 Vaccine of Toxoplasma gondii

Objective To construct recombinant Escherichia coli/Mycobacterium shuttle plasmid pSTM3-p30-Rop2 of Toxoplasma gondii. Methods p30-Rop2 compound gene was amplified from pET30-p30-Rop2 and subcloned into the shuttle plasmid pSTM3 to attain a recombinant plasmid pSTM3-p30-Rop2. Results p30-Rop2 compound gene was subcloned into pSTM3 correctly identified by digestion . There was a mutation analyzed by sequencing but did not change the open reading frame. Conclusion The recombinant M. S-pSTM3-p30-Rop2 is constructed successfully which lay a foundation to the construct of new vaccine of Toxoplasma gondii.