| 日本血吸虫未成熟虫卵26-28kDa抗原的分离与纯化 |
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| 引用本文： | 汪世平,周汨波,李华,曾宪芳,赵慰先.日本血吸虫未成熟虫卵26-28kDa抗原的分离与纯化[J].中国血吸虫病防治杂志,1996(5). |
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| 作者姓名： | 汪世平 周汨波 李华 曾宪芳 赵慰先 |
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| 作者单位： | 湖南医科大学!长沙410078(汪世平，周汨波，李华，曾宪芳)，南京医科大学(赵慰先) |
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| 基金项目： | 国家自然科学基金，卫生部血吸虫病重点研究项目(总理基金)资助 |
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| 摘 要： | 本文采用超凝胶AcA柱层析方法和SDS-PAGE结合凝胶洗脱方法等,分离纯化了日本血吸虫未成熟虫卵可溶性抗原(SIEA)26-28kDa组分.SDS-PAGE、免疫印迹分析(EITB)和银染色等证明,采用该方法分离纯化的26-28kDa抗原纯度高,有较强的免疫活性,并证明该抗原组分在SIEA中含量丰富.纯化的SIEA26-28kDa可作为抗日本血吸虫生殖产卵和抗卵胚发育侯选抗原,用于抗病保护性免疫的研究.
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| 关 键 词： | 日本血吸虫 未成熟虫卵 26-28kDa 抗原 纯化 |
ISOLATION AND PURIFICATION OF 26-28kDa ANTIGENS FROM IMMATURE EGGS OF SCHISTOSOMA JAPONICUM |
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| Wang Shiping Zhou Mibo Li Hua Zeng XianfangHunan Medical University.ISOLATION AND PURIFICATION OF 26-28kDa ANTIGENS FROM IMMATURE EGGS OF SCHISTOSOMA JAPONICUM[J].Chinese Journal of Schistosomiasis Control,1996(5). |
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| Authors: | Wang Shiping Zhou Mibo Li Hua Zeng XianfangHunan Medical University |
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| Institution: | Changsha 410078 |
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| Abstract: | A protective 26-28kDa antigen was purified from japonicum the supernatant of immature egg homogenate of Schistosoma japonicum using AcA ultragel chromatography and SDS-PAGE. The results of SDS-PAGE followed by silver. stain and EITB indicated that the isolated 26-28kDa antigen was purificatory both biochemically and immunologically. It was also proved that the 26-28kDa molecule is one of the major antigens in SIEA. The important functions of the 26-28kDa antigen from SIEA in anti-fecundity and anti-embryonation immunity against Schistosoma japonicum were discussed. |
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| Keywords: | Schistosoma japonicum Immature egg 26-28kDa antigen purification chromatography |
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