碘海醇对实验性大鼠草酸钙结石形成的影响

目的探讨造影剂碘海醇对大鼠草酸钙结石形成的影响。方法将40只Wister大鼠随机分为成4组：空白对照组（A）、单纯诱石组（B）、单纯碘海醇组（C）、诱石＋碘海醇组（D）。用乙二醇法诱导建立大鼠草酸钙结石模型，C、D组尾静脉注射碘海醇。实验第4周末检测肾组织中丙二醛（MDA）含量及超氧化物歧化酶（SOD）活力，镜下观察肾内草酸钙晶体沉积情况。结果A、B、C、D4组肾脏组织MDA含量（nmol／mg蛋白）分别为2．74±0．48、4．51±1．47、6．92±2．18、8．87±2．31，SOD活力（U／mg蛋白）分别195．21±11．01、170．24±13．59、140．86±25．91、121．54±25．50，各组间差异有统计学意义（P〈0．05）。B、D组结晶等级评分高于A组，D组结晶等级评分高于B组（P〈1．05）。培论碘海醇促进了大鼠肾小管内草酸结晶量的增加，其机制与碘海醇诱导的肾小管上皮氧化应激损伤有关。

Effects of iohexol on experimental calcium oxalate crystallization formation in rats

Objective To investigate the effects of iohexol on experimental calcium oxalate crystallization in rats. Meth- ods A total of 40 rats were randomly divided into 4 groups： blank control group （A）, stone forming group （B）, iohexol group （C）, and stone forming and iohexol group （D）. Calcium oxalate crystals were produced in rats by ethylene glycol. After 4 weeks the rats were sacrificed and the renal tissues were removed to determine malonaldehyde （MDA） content and superoxide dismutase （SOD） activities. Pathological changes in renal tissues were observed with light microscopes. Results The MDA contents（nmol/mg） in renal tissues were 2.74±0.48, 4. 51±1.47, 6. 924±2.18, 8. 875：2.31, and SOD activities（U/mg） were 195.21±11.01, 170.24±13.59, 140.86±25.91, 121.54±25.50. Compared with group A, the grade of calcium oxalate crys- tallization was significantly increased in group B and D （P〈O. 05）. Compared with group B, the grade of calcium oxalate crys- tallization was significantly increased in group D （P〈O. 05）. Conclusions Iohexol induced the increase of calcium oxalate crystallization. The mechanism is connected with the oxidative damage of renal tubule epithelial ceils.