天然14肽生长抑素联合5-Fu对胃癌移植瘤的抑制作用

目的 观察14肽生长抑素(SST-14)及其联合5-Fu对BGC-823、MKN-28人胃癌细胞移植瘤生长抑制及血管内皮生长因子(VEGF)表达的影响。方法 构建BGC-823、MKN-28人胃癌细胞移植瘤模型,随机分为对照组、SST-14组、5-Fu组、联合组,腹腔注射药物治疗3周后处死裸鼠获取肿瘤组织。测量瘤体重量,计算抑瘤率;免疫组化及Western blot检测瘤组织VEGF的表达。结果 其他三组较对照组均可抑制肿瘤生长,联合组较SST-14、5-Fu单纯用药组抑制作用显著(P＜0.05)。免疫组化及Western blot结果显示,与对照组相比,其他三组瘤组织VEGF表达下降(P＜0.05);其中联合组较SST-14组、5-Fu组显著下降(P＜0.05)。结论 14肽生长抑素联合5-Fu能够有效抑制不同分化程度胃癌细胞移植瘤生长,且优于单纯用药组,两药具有协同抑制作用,可能通过下调VEGF的表达,抑制肿瘤血管生成。

Effect of somatostatin-14 combined with 5-Fu on suppression in human gastric cancer xenografts in nude mice

Objective To observe the effect of Somatostatin-14(SST-14)and SST-14 combined with 5-Fu on the suppressive growth and vessculor endothelial growth factor(VEGF)expression in nude mice subcutaneous xenografts.Methods The animal models of xenotransplanted human gastric cancer were established by implanting BGC-823 and MKN-28 cell lines subcutaneously in nude mice and were randomly divided into 4 groups and treated with normal saline,SST-14,5-Fu and SST-14 combined with 5-Fu for three weeks,respectively.Three weeks after treatment,the animals were killed to take the tumors.The tumor growth inhibition was used to evaluate the suppressive growth.Immunohistochemistry and Western blot were applied to observe the expression of VEGF.Results SST-14 combined with 5-Fu obviously reduced the tumor weight of xenografts(P＜0.05).Immunohistochemical and Western blot indicated that VEGF expression and positive cells in three treatment groups were significantly decreased compared with normal saline group(P＜0.05).Moreover,VEGF expression in combined treatment group was reduced significantly than SST-14,5-Fu groups(P＜0.05).Conclusion Somatostatin-14 combined with 5-Fu significantly suppresses the subcutaneous xenografts growth in nude mice by different human gastric cancer cell lines.The inhibition of angiogenesis by SST-14 together with 5-Fu may through down-regulating the expression of VEGF in subcutaneous xenografts in nude mice.