人肝癌裸小鼠模型的建立及其生物学特性的研究 |
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引用本文: | 刘锡忠 胡敬群. 人肝癌裸小鼠模型的建立及其生物学特性的研究[J]. 中国医学科学院学报, 1995, 17(2): 98-103 |
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作者姓名: | 刘锡忠 胡敬群 |
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作者单位: | 中国医学科学院,中国协和医科大学肿瘤研究所 |
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摘 要: | 将7例原发性肝癌手术切除标一分别移植于BALB/c裸小鼠皮下,1例获得成功,并能在裸鼠体内连续传代。11个月内已传了10代。其潜伏期为11d左右,荷瘤鼠血清可测得甲脂蛋白、γ-谷氨酰转肽酶和酸性同工铁蛋白三种有意义的肝癌血清标记物。移植癌病理结构与瘤原相似,其遗传学特征与人类恶性肿瘤相同。
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关 键 词: | 裸鼠模型 体外细胞培养 肝肿瘤 |
Establishment and Biological Characteristics of the Nude Mice Xenograft Model from Human Hepatocellular Carcinoma |
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Affiliation: | (Cancer Institute,CAMS and PUMC,Beijing 100021) |
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Abstract: | The human hepatocellular carcinoma was successfully heterotrasplanted into nude mice and 10 passages were made seriously during 11 months,InitiaI take rate was 14.3%and then the take rate increased to 1 00?ter the 3rd passage.Histological,morphological,ultrastructural and biological investigation and chromosome analysis showed that the characteristic of transplanted tumor was identical to that of characteristics of human donor tumor.Through serial passages no metastatic signs were found grossly and under the microscope in the lymph nodes,liver and lung of the nude mice,Result also showed that the model retained some functions of the originaI tumor,The transplanted tumor could secret a-fetoprotein(AFP),r-glufamyl transpeptidase(r-GT).Acidic isoferritin(AIF)also could be detected.The tumor tissue of xenograft model was cultured in vitro in order to develop a permanent cell line.We found that the tumor specimen of the later passages grew more rapidly than the earlier ones,Up to now 3 cell passages were made.In this paper,a reliable animal model of human hepatocellular carcinoma is provided for studies of clinical diagnosis,treatment,antitumor drug screen test,etc. |
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Keywords: | human hepatocellular carcinoma xenograft nude mice cell culture |
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