荧光原位杂交技术检测血管免疫母细胞性淋巴结病3号染色体三体畸变

运用荧光原位杂交技术，检测３６例血管免疫母细胞性淋巴结病（ＡＩＬＤ）中常见的３号染色体三体（＋３）的发生率及含有＋３的畸变细胞率。结果２８例（７７．８％）ＡＩＬＤ有＋３，远比细胞遗传学检查发现的１５例（４２．０％）为多；而含有＋３的畸变细胞率平均为４．０％，比染色体分析发现的１１．２％低。本研究表明，＋３是ＡＩＬＤ极为特异性的染色体畸变；含有＋３的肿瘤细胞仅占少数，并具有生长优势；荧光原位杂交技术在检测染色体数目畸变方面远较传统的细胞遗传学方法敏感。

Detection of Trisomy 3 in Angioimmunoblastic Lymphadenopathy with Dysproteinemia by Fluorescence in Situ Hybridization

Using fluorescence in situ hybridization, we have detected trisomy 3 and the frequency of cells with 3in 36 patients with AILD.In 36 cases 28(77.8%)were found having tri somy 3,which was far exceeded that observed in metaphase cytogenetic study( 3 in 15 cases,42%).Meanwhile,the frequency of cells with 3 was only 4.0%, which was much lower than that of 11.2%in metaphase cytogenetics.The study indicate that the trisomy 3 is a characteristic chromosome aberration of AILD,the cells with 3 constitute only a small part of the specimen,the fluorescence in situ hybridization is more sensitive than metaphase cytogenetics in detection of numerical chromosome aberrations.