饱和氢盐水减轻大鼠急性放射性脑损伤早期的氧化应激

目的 探讨饱和氢盐水对电离辐射诱发的脑组织损伤的保护作用。方法 成年4月龄健康SD大鼠45只,采用随机数字表法分为健康对照组,盐水治疗组和氢水治疗组,再根据照射后时间分为1、3、7和14 d 4 个时间组,每组5只。4 MeV电子线对大鼠进行单次垂直全脑照射,吸收剂量率为200 cGy/min,吸收剂量20 Gy。氢水治疗组大鼠于照射后即刻、照后连续3 d给予饱和氢盐水腹腔注射,分别于照射后1、3、7和14 d处死大鼠,取脑组织制作匀浆,分光光度计检测超氧化物歧化酶(SOD)、丙二醛(MDA)、8-羟基脱氧鸟嘌呤(8-OHdG)含量。HE染色观察海马区脑组织病理变化。结果 7和14 d时氢水治疗组脑组织含水量明显小于盐水治疗组(t=3.78、3.18, P<0.05),SOD水平1~7 d时氢水治疗组明显高于盐水治疗组(t=2.41~2.92,P<0.05),MDA水平明显低于盐水治疗组(t=4.01~6.03,P<0.05),持续到14 d,8-OHdG含量1~7 d氢水治疗组明显低于盐水治疗组(t=2.33、2.71、2.33,P<0.05)。海马区神经细胞损伤明显轻于对照组。结论 饱和氢盐水对大鼠急性放射性脑损伤早期有保护作用。

Hydrogen-rich saline alleviation on the oxidative stress and early-phase radiation-induced brain injury in rats

Objective To investigate the effect of hydrogen on radiation-induced acute injury in rat brain.Methods Forty-five mature Sprague-Dawley rats were randomly divided into three groups: saline therapy group, hydrogen therapy group and healthy control group. The whole brain of SD rat was irradiated with single dose of 20 Gy by 4 MeV electrons. Rats in therapy group were injected with hydrogen-rich saline after irradiation and were sacrificed at 1, 3, 7, 14 d post-irradiation. The changes of malonaldehyde (MDA), superoxidase dismutase (SOD) and 8-hydroxydeoxygunosine (8-OHdG) in brain homogenate and the pathological changes in brain hippocampus were observed. Results The brain water content (t=3.78,3.18,P<0.05) and the contents of 8-OHdG (t=2.33, 2.71, 2.33,P<0.05) in the therapy group was lower than the control group at 7 d and 14 d post-irradiation. The contents of SOD were significantly higher(t=2.41-2.92, P<0.05)from 1 to 7 day, while the contents of MDA were significantly lower in therapy group than those in the control group from 1 to 14 day post-irradiation (t=4.01-6.20, P<0.05). Moreover, the damage degree in the nerve cells of hippocampus was less compared to the control group. Conclusions The hydrogen-rich saline could have protection role in irradiation-induced acute brain injury in rats.