腹腔肝细胞移植治疗大鼠急性肝功能衰竭

目的观察腹腔肝细胞移植（HCT）治疗大鼠急性肝功能衰竭（ALF）疗效。方法取10只大鼠用胶原酶消化法分离肝细胞；另取50只大鼠随机分为A组20只、B组20只、C组10只。A、B组均采用D-氨基半乳糖（D-GalN）制作ALF模型，造模后分别腹腔注射肝细胞悬液2．5ml／只、不含肝细胞的培养液2．5ml／只；C组不予任何处理。观察移植后48h肝功能情况、肝脏病理改变及7d存活率。结果A、B组ALT、AST、AMM均高于C组（P均〈0．01），但A、B组间比较，P〉0．05；A组7d存活率为50．00％，B组为14．28％，两组比较，P〈0．01。A组7d肝小叶结构基本恢复正常，仅见少量肝细胞脂肪样变性，汇管区见少量炎性细胞；B组肝小叶结构仍紊乱，较多细胞呈脂肪样变性，可见较多炎性细胞浸润。结论经腹腔HCT治疗D—GalN诱导的大鼠ALF有效。

Effect of hepatocytes transplantation through abdominal injection in rats with acute liver failure

Objective To observe the effect of hepatocyte transplantation （HCT） through abdominal injection in rats with acute liver failure （ALF）. Methods Rat hepatocytes were isolated with collagenase digestion. ALF rats were induced by D-GalN. HCTs were performed 24 h after the models were established. Rats were distributed in HCT group（ Group A ,n = 20） , model control group （ Group B, n = 20） , angroup （ group C, n = 10） at random. Group A received 2.5 × 10^7 hepatocytes for every rat by abdominal injection. The 48 h liver function after HCT was assessed in group A, B and C. The 7 day survival rate after HCT was observed in group A dnormal control and B. Liver histology was observed for all rats. Result The survival rates of isolated hepatocytes was 87% - 95%. About 7.8 × 10^7 hepatocytes could be isolated in one rat. Group A and B showed a higher serum GPT, GOT and AMM significantly than that of group E （ P 〈 0.01 ） ; but there was no difference between group A and B （ P 〉 0.05 ）. The 7 day survival rate of group C was much higher than that of group D （50.00% vs 14.28% ,P 〈0.01 ）. The liver histology was improved in group A,compared with group B 7 d after HCT. Conclusion HCT through abdominal injection is effective for ALF induced by D-GalN in rats.