遗尿方对肾阳虚多尿大鼠抗利尿激素含量和环磷酸腺苷、环磷酸鸟苷比例的影响

目的：探讨遗尿方对肾阳虚多尿大鼠的影响。 方法：40只雄性Wistar大鼠随机分为空白对照组、模型组、去氨加压素（弥凝）组、小剂量遗尿方组和大剂量遗尿方组，每组8只。空白组予生理盐水0.2 mL肌注，其余组给予氢化可的松25 mg/kg肌注造模，1次/d，连续21 d；造模第8天起分别给予双蒸水、弥凝、大剂量遗尿方、小剂量遗尿方灌胃30 d。观察治疗前后24 h尿量改变，治疗后血清抗利尿激素——精氨酸加压素（arginine vasopressin，AVP）含量，血浆环磷酸腺苷（cyclic adenosine monophosphate，cAMP）、环磷酸鸟苷（cyclic guanosine monophosphate，cGMP）含量，cAMP/cGMP比值及肾组织的形态学改变。 结果：与空白对照组比较，模型组24 h尿量明显增多，血清AVP含量及血浆cAMP/cGMP比值明显降低；与模型组比较，各用药组尿量减少，血清AVP含量及血浆cAMP/cGMP比值明显升高（P〈0.05，P〈0.01）。各组肾组织病理改变不明显。 结论：遗尿方治疗肾阳虚多尿大鼠的作用机制可能与升高血清AVP含量和血浆cAMP/cGMP比值有关。

Effects of Yiniao Recipe on serum antidiuretic hormone level and plasma ratio of cAMP to cGMP in rats with kidney-yang deficiency

Objective： To investigate the effects of Yiniao Recipe, a compound traditional Chinese herbal medicine, on contents of serum antidiuretic hormone, and plasma cyclic adenosine monophosphate and cyclic guanosine monophosphate in rats with kidney-yang deficiency.
Methods： Forty male Wistar rats were randomly divided into blank control group, untreated group, desmopressin （Minirin） group, low-dose Yiniao Recipe group and high-dose Yiniao Recipe group, with 8 rats in each group. Rats in the blank control group were injected with 0.2 mL normal saline, and rats in the other groups were given intramuscular injection of hydrocortisone 25 mg/kg, 1 time daily for 21 consecutive days; from the 8th day of injection, rats were given double distilled water, Minirin, and high- and low-dose Yiniao Recipe respectively for 30 days. Before and after treatment, 24-hour urine volume was observed, and serum antidiuretic hormone （AVP） as well as plasma cyclic adenosine monophosphate （cAMP） and cyclic guanosine monophosphate （cGMP） contents were detected by enzyme-linked immunosorbent assay. The cAMP/cGMP ratio and morphological changes in renal tissues were also observed.
Results： Compared with blank control group, 24-hour urine volume, serum AVP content and cAMP/cGMP ratio in the untreated group were decreased; compared with the untreated group, Minirin and Yiniao Recipe at low and high doses reduced 24-hour urine volume and increased serum AVP content and cAMP/cGMP ratio significantly （P〈0.05 or P〈0.01）. There were no obvious pathological changes in renal tissue in all groups.
Conclusion： Yiniao Recipe may reduce 24-hour urine volume by increasing serum AVP content and regulating the ratio of cAMP to cGMP in kidney-yang deficiency rats.