黄连解毒汤及其各部位群抗炎物质基础

目的:研究黄连解毒汤(HLJDT)全方及其各部位群抗炎活性,讨论HLJDT抗炎活性的物质基础。方法:通过鼠毛细管通透性和二甲苯致炎实验,建立动物模型以及确定黄连解毒汤的有效剂量,并依据全方的有效剂量计算出各组分群的给药剂量。将动物随机分为空白组,模型组,地塞米松组,HLJDT全方组,HLJDT上清液50%醇,HLJDT沉淀水洗,沉淀50%醇洗组,按传统给药方式连续3 d ig给药。末次给药60 min后尾静脉注射伊文思蓝(EB),然后立即进行小鼠二甲苯致炎实验,结合高效液相色谱(HPLC)研究HLJDT全方及其各部位群抗炎作用,比较各组间差异。结果:地塞米松,HLJDT全方,上清液50%醇洗和沉淀50%醇洗均能有效降低模型组的肿胀度,与模型组比较有显著性差异(P0.01)。HLJDT全方,上清液50%醇洗和沉淀50%醇洗部位群均能有效降低耳片中EB含量,抑制肿胀度,EB含量分别为(11.8±1.8),(23.4±3.8),(25.2±3.2)ng·g-1;肿胀度抑制率分别为26.44%,29.89%,19.54%;HLJDT上清液50%醇洗抗炎作用比沉淀50%醇洗作用显著。结论:HLJDT,上清液50%醇洗和沉淀50%醇洗均能有效降低模型组的肿胀度,结合HPLC结果可推断,黄酮与生物碱相互作用后,可以降低生物碱副作用,增加环烯醚萜类成分后,抗炎效果更显著,为抗炎有效部位。

Anti-inflammatory Material Basis of Huanglian Jiedu Tang and Its Fractions

Objective: To study the anti-inflammatory activity of Huanglian Jiedu Tang(HLJDT) and its fractions as well as its material basis. Method: Xylene-induced ear-swelling and capillary permeability experiments were carried out to build the animal model, in order to determine the effective dose of HLJDT and then calculate the dose of each fractions of HLJDT. The animals were randomly divided into the blank group, the model group, the dexamethasone group, the HLJDT group, the HLJDT 50% alcohol-treated supernatant group, the water-treated precipitation group and the 50% alcohol-treated precipitation group, and administrated with drugs according to the traditional way for 3 days. Evans blue(EB) was intravenously injected 60 min later after the last administration, and then the mouse xylene induced inflammation experiment was immediately carried out. The anti-inflammatory effect of HLJDT and its fractions was researched with high performance liquid chromatography(HPLC), and inter-group differences were compared. Result: Compared with the model group, dexamethasone, HLJDT, HLJDT 50% alcohol-treated supernatant and 50% alcohol-treated precipitation could effectively reduce the swelling degree and showed significant difference(P<0.01). The content of EB in the three groups mentioned above decreased to(11.8±1.8),(23.4±3.8),(25.2±3.2) ng·g^-1, the swelling inhibition rates were 26.44%, 29.89%, 19.54%. HLJDT 50% alcohol-treated supernatant showed a more significant effect on anti-inflammatory than 50% alcohol-treated precipitation. Conclusion: HLJDT, HLJDT 50% alcohol-treated supernatant and 50% alcohol-treated precipitation could effectively reduce the swelling degree. On the basis of the result of HPLC, it can be inferred that the flavonoids interacted with alkaloids to reduce the side effect of alkaloids, increase the iridoids and showed a significant anti-inflammatory effect. Therefore, they are the effective anti-inflammatory fractions.