基于指纹图谱的决明子有效组分纯化工艺

目的:采用HPLC指纹图谱指导优化决明子有效组分纯化工艺。方法:采用HPLC建立决明子指纹图谱,以5个主要峰的回收率和指纹图谱相似度为指标,优选大孔树脂型号、上样量和洗脱条件。结果:D-101树脂对5个成分的回收率最高,上样量为3 m L,树脂用量为10 m L,洗脱溶剂为75%乙醇50 m L,纯化后主峰回收率均85%,RSD均0.6%,HPLC指纹图谱相似度0.98。结论:采用HPLC指纹图谱指导优化决明子有效组分大孔树脂纯化工艺,可保持分离前后有效组分中化学成分的一致性。

Purification Technology of Active Materiomic from Cassiae Semen Based on Fingerprint

Objective: To optimize the technological parameters in the purification process of active materiomic from Cassia Semen by HPLC fingerprint technique. Method: Using the established HPLC fingerprint of Cassia Semen, the macroporous resin type, loaded concentrations, and elution conditions were optimized using the similarity and recovery rates of five components in fingerprints as indexes. Result: D-101 type resin possessed the highest recovery rates of five components. The optimized purification process was as follows:the loaded concentration was 3 mL, the amount of resin was 10 mL, 50 mL of 75% ethanol was used as eluent, respectively. After being purified, the recovery rates of five components were all over 85%, and the similarity of fingerprints was higher than 0.98.These components could get balanced recovery. Conclusion: Based on HPLC fingerprint technique, the optimized purification process of active materiomic from Cassiae Semen with macroporous resin can ensure that the main effective components are consistent with the crude extract of Cassiae Semen.