Quantitative assessment of BAX transcript and flow cytometric expression in acute myeloid leukemia: A prospective study |
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| Authors: | Surender Kumar Sharawat Vinod Raina Lalit Kumar Atul Sharma Radhika Bakhshi Sreenivas Vishnubhatla |
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| Affiliation: | 1. Department of Medical OncologyDr B. R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India;2. Department of Biomedical SciencesShaheed Rajguru College of Applied Sciences, University of Delhi, New Delhi, India;3. Department of BiostatisticsAll India Institute of Medical Sciences, New Delhi, India |
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| Abstract: | ObjectiveQuantitative assessment of BAX transcripts and protein in acute myeloid leukemia (AML).MethodsWe quantitatively evaluated BAX gene transcripts by real-time polymerase chain reaction (TaqMan probe chemistry) and protein expression by flow cytometry.ResultsConsecutive 112 AML patients with a median age of 16 (1–59) years were recruited in the study. By flow cytometry, the percentage expression was in linear correlation with relative median fluorescent intensity (RMFI; R = 0.4425; P < 0.001). However, there was no linear relationship between the transcript copies of the BAX with its RMFI (R = ?0.0559; P = 0.586). The expression of the BAX at both protein and transcript level was significantly higher in AML patients as compared with normal control. RMFI of the BAX were higher in the cohort with lower white blood cell count (P = 0.029). None of the other baseline characteristics correlated with either the BAX transcript or the RMFI. BAX expression did not correlate with complete remission rate, event free, disease free, and overall survival.ConclusionBAX gene expression in AML was evaluated first time with two different methods but did not correlate with the survival outcome |
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| Keywords: | Acute myeloid leukemia BAX Flow cytometry Real-time PCR Taq Man Probe Prognosis |
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