运动对2型糖尿病小鼠内皮祖细胞功能的影响

目的 探讨不同运动方式对2型糖尿病小鼠内皮祖细胞(EPCs)功能的影响,明确2型糖尿病小鼠预防心血管并发症的最佳运动方式。方法 8周龄雄性2型糖尿病小鼠随机分为4组:对照组、有氧运动组、抗阻运动组、联合运动组。对照组不作运动干预;有氧运动组进行无负重跑步运动;抗阻运动组进行尾部负重爬梯运动;联合运动组隔日交替进行有氧运动和抗阻运动。运动干预1 、2 、4 周时处死小鼠,体外分离培养骨髓源性EPCs并检测其增殖、粘附、迁移及血管生成能力。结果 组间比较:运动干预4周时,各运动组EPCs粘附、迁移及血管生成能力均强于对照组,以联合运动效果最优。组内比较:各运动组EPCs粘附、迁移和血管生成能力在运动干预第2周时强于第1周时,增殖能力在第4周时强于第1周时(均P<0.05)。结论 与有氧运动和抗阻运动相比,联合运动在预防2型糖尿病小鼠心血管并发症方面效果更佳,运动效果随着时间的延长而增强。

Effect of sports training on functions of endothelial progenitor cells in mice with type 2 diabetes

Objective To explore the effects of different exercise modes on functions of endothelial progenitor cells(EPCs)and to find out the best way to prevent cardiovascular complications in mice with type 2 diabetes.Methods Male mice with type 2 diabetes aged 8 weeks were randomly divided into control group,aerobic training(AT)group,resistant training(RT)group and AT+RT group.Mice in the control group were kept sedentary.Mice in AT group performed treadmill running.Burdened with a certain weight,mice in the RT group climbed the man-made ladder.Mice in AT+RT group performed aerobic training and resistant training in turn.Mice were killed at the 1st,2nd,4th week of intervention.Bone marrow-derived EPCs were isolated and its functions of proliferation,adhesion,migration and angiogenesis were identified.Results Compared with the control group,the EPCs in AT,RT and AT+RT showed better abilities to adhere,migrate and generate new vessel at the end of 4th week,among which AT+RT showed the best ability.Improvement in the adhesion,migration and angiogenesis of the EPCs were observed at the end of 2rd than that at 1st week(P<0.05)and improvement in proliferative ability was observed at the end of the 4th week then that at 1st week(P<0.05).Conclusion Compared to aerobic training or resistant training,combined aerobic and resistant training is more effective for preventing cardiovascular complications in mice with type 2 diabetes and the positive effects enhanced with the extension of exercise time.