| 反相高效液相色谱法测定清开灵注射液中2组分的含量 |
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| 引用本文： | 赵绪元,姚金成,胡领,韩晓璐.反相高效液相色谱法测定清开灵注射液中2组分的含量[J].中国药房,2007,18(3):202-203. |
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| 作者姓名： | 赵绪元 姚金成 胡领 韩晓璐 |
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| 作者单位： | 1. 中南大学湘雅二医院临床药学研究室,长沙市,410011
2. 中国药科大学国家新药筛选中心,南京市,210009 |
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| 摘 要： | 目的建立以反相高效液相色谱法同时测定清开灵注射液中黄芩苷、栀子苷含量的方法。方法色谱柱为DiamonsilTMC18(250mm×4.6mm,5μm),流动相为乙腈-20mmol.L-1磷酸二氢钠溶液(20∶80),流速为1.0mL.min-1,检测波长为238nm。结果黄芩苷在1~50μg.mL-1浓度范围内线性良好(r=0.9995),平均回收率为100.75%(RSD=1.75%);栀子苷在0.1~10μg.mL-1浓度范围内线性良好(r=0.9997),平均回收率为100.63%(RSD=1.35%)。结论本方法快速、简便、准确、灵敏度高,可用于清开灵注射液的质量控制。
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| 关 键 词： | 反相高效液相色谱法 清开灵注射液 黄芩苷 栀子苷 含量测定 |
| 文章编号： | 1001-0408（2007）03-0202-02 |
| 收稿时间： | 2006-04-18 |
| 修稿时间： | 2006-07-01 |
Determination of Baicalin and Jasminoidin in Qingkailing Injection by RP-HPLC |
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| ZHAO Xuyuan,YAO Jincheng,HU Ling,HAN Xiaolu.Determination of Baicalin and Jasminoidin in Qingkailing Injection by RP-HPLC[J].China Pharmacy,2007,18(3):202-203. |
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| Authors: | ZHAO Xuyuan YAO Jincheng HU Ling HAN Xiaolu |
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| Abstract: | OBJECTIVE:To establish a RP-HPLC method for the determination of baicalin and jasminoidin in Qingkailing Injection.METHODS:It was performed on DiamonsilTM C18 column with acetonitrile-20 mmol·L-1 sodium dihydrogen phosphate solution (20:80)as mobile phase.The flow rate was 1.0 mL·min-1.The detection wavelength was 238 nm.RESULTS:The liner range of baicalin was 1～50μg·mL-1,(r=0.999 5)and the average recovery was 100.75%(RSD=1.75%);The liner range of jasminoidin was 0.1～10μg·mL-1(r=0.999 7)and the average recovery was 100.63%(RSD=1.35%).CONCLUSION:This method is rapid,simple,accurate and sensitive.It can be used for quality control of Qingkailing Injection. |
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| Keywords: | RP-HPLC Qingkailing injection Baicalin Jasminoidin Assaying |
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