牛奶和奶粉中八种镇定剂残留量的液相色谱-串联质谱测定

采用液相色谱-串联质谱仪,建立了牛奶和奶粉中乙酰丙嗪cetopromaizine、氯丙嗪chlor-promazin、氟哌啶醇haloperidol、丙酰二甲氨基丙吩噻嗪propionylpromazine、甲苯噻嗪xylazine、阿扎哌隆azaperone及其代谢物阿扎哌醇azaperol和咔唑心安carazolol残留量的液相色谱-串联质谱测定方法。结果表明八种镇定剂在牛奶中添加水平0.10～0.50μg/kg到0.80～4.00μg/kg,其回收率在86.29%～92.49%之间,相对标准偏差在8.99%～20.56%之间;在奶粉中添加水平0.80～2.00μg/kg到2.0～8.00μg/kg,其回收率在81.59%～97.49%之间,相对标准偏差在1.76%～16.70%之间。八种镇定剂在确定的添加范围内线性关系良好。八种镇定剂在牛奶中的方法检出限为0.1～0.5μg/kg,在奶粉中的方法检出限为0.8～4.0μg/kg。

Determination of eight tranquillizers residues in bovine milk and milk powder-LC-MS-MS

LC-MS-MS method was established for the determination of cetopromaizine chlorpromazin,haloperidol,propionylpromazine,xylazine,azaperone,azaperol and carazolol residues in bovine milk and milk powder.The result showed that the average spiked recoveries of eight tranquillizers at the spiked level 0.10～0.50 μg/kg to 0.80～4.00 μg/kg were from 86.29%～92.49% with relative standard deviations（RSDS） of 8.99%～20.56% for bovine milk and at the spiked level 0.80～2.00 μg/kg to 2.0～8.00 μg/kg were from81.59%～97.49% with relative standard deviations（RSDS） of 1.76%～16.70% for milk powder.The result showed a good linea relationship.The limits of determination of method for eight tranguillizers were from 0.10 μg/kg to 0.50 μg/kg for bovine milk and 0.8 μg/kg to 4.0 μg/kg for milk powder.