| RP-HPLC测定地稔药材中没食子酸的含量 |
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| 引用本文： | 何迅李勇军,刘丽娜,兰燕宇,王爱民,王永林. RP-HPLC测定地稔药材中没食子酸的含量[J]. 中国中药杂志, 2005, 30(3): 180-181 |
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| 作者姓名： | 何迅李勇军 刘丽娜 兰燕宇 王爱民 王永林 |
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| 作者单位： | 贵阳医学院,药学院,贵州,贵阳,550004 |
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| 摘 要： | 目的建立地稔药材中没食子酸的含量测定方法,为其质量标准的建立提供科学依据。方法采用RPHPLC直接测定,色谱柱DiamonsilC18柱,流动相为四氢呋喃甲醇02%磷酸(05∶05∶99),流速1mL·min-1,检测波长274nm。结果测定了10批不同地区市售的地稔药材,没食子酸含量在0020%～0081%。结论本方法简便、快速、准确,可作为地稔药材的质量检测方法。
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| 关 键 词： | 地稔 没食子酸 RP-HPLC |
| 文章编号： | 1001-5302(2005)03-0180-02 |
| 收稿时间： | 2004-01-11 |
Determination of gallic acid in Melastoma dodecandrum by RP-HPLC |
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| HE Xun;LI Yong-jun; LIU Li-na; LAN Yan-yu; WANG Ai-min;WANG Yong-lin. Determination of gallic acid in Melastoma dodecandrum by RP-HPLC[J]. China Journal of Chinese Materia Medica, 2005, 30(3): 180-181 |
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| Authors: | HE Xun LI Yong-jun LIU Li-na LAN Yan-yu WANG Ai-min WANG Yong-lin |
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| Affiliation: | School of Pharmacy, Guiyang Medical College, Guiyang 550004, China. hexun224@gmc.edu.cn |
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| Abstract: | OBJECTIVE: To establish a RP-HPLC method for the determination of gallic acid in Chinese herb Melastoma dodecandrum, and to evaluate the quality of the herb. METHOD: The RP-HPLC analysis was achieved by using a Polaris C18 column and tetrahydrofuran-methanol-0.2% phosphoric acid (0.5:0.5:99) as the mobile phase, with a flow rate of 1.0 mL x min(-1), and detected by UV at 274 nm. RESULT: The content of gallic acid was from 0.020% to 0.081%, in ten groups of M. dodecandrum collected from different locations. CONCLUSION: The method is a simple, convenient and rapid. It can be used for quality evaluation of M. dodecandrum. |
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| Keywords: | Melastoma dodecandrum gallic acid RP-HPLC |
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