甲状旁腺激素联合降钙素对大鼠骨质疏松性骨折骨生长因子的影响

目的探讨甲状旁腺素联合降钙素对大鼠骨质疏松性骨折骨相关生长因子及骨折愈合的影响。方法75只雌性SD大鼠随机分为:假手术组、去势组、甲状旁腺素组、降钙素组、联合用药组,15只/组,首先构建去卵巢大鼠骨质疏松性骨折动物模型。观察并评估骨折愈合,测定骨痂BMD和BMC,检测血清BMP-2、VEGF、TGF-β、IGF-1水平和骨痂蛋白表达,观察骨痂形态结构变化。结果去势组较假手术组骨折愈合评分、骨痂BMD和BMC、血清BMP-2、VEGF、TGF-β、IGF-1水平和骨痂蛋白表达均显著降低(P均<0.05),而联合用药组较去势组上述指标均显著升高(P均<0.05)。去势组骨痂骨小梁明显减少,生长稀疏,排列紊乱,大量间充质干细胞及纤维软骨细胞,成骨细胞及微血管少见;联合用药组骨痂大量骨小梁,生长旺盛,互相交错网状,排列致密有序,可见较多成熟的骨细胞及成骨细胞。结论甲状旁腺素联合降钙素通过介导提高相关骨生长因子表达,提高骨质疏松性骨折骨密度和矿物含量,改善骨组织形态学,加快骨折愈合。

Effect of parathyroid hormone combined with calcitonin on bone growth factor in rats with osteoporotic fracture

Objective To study the effect of parathyroid hormone combined with calcitonin on bone-related growth factor and fracture healing in rats with osteoporotic fracture.Methods 75 female SD rats were randomly divided into:sham operation group,ovariectomized group,parathyroidism group,calcitonin group,and combination drug group,with 15 rats in each group.Firstly,an animal model of osteoporotic fracture in ovariectomized rats was constructed.Fracture healing was observes and evaluated,osteophyte BMD and BMC were determined,serum BMP-2,VEGF,TGF-β,IGF-1 concentration and osteopontin expression were assessed,and the morphology of the bone callus was observed.Results Compared with the sham operation group,the fracture healing score,BMD and BMC,serum BMP-2,VEGF,TGF-β,IGF-1 and osteopontin expression were significantly lower in the ovariectomized group(P<0.05).The above-mentioned indicators in the combination group were significantly higher than the ovariectomized group(P<0.05).In the ovariectomized group,the trabecular bone of the osteophyte was significantly reduced,the growth was sparse,the arrangement was disordered,there was a large number of mesenchymal stem cells and fibrocartilage cells,whereas osteoblasts and microvessels were rare.The combination group had a large number of trabecular bones,which grew vigorously,and were interlaced with each other.The arrangement was dense and orderly,and more mature bone cells and osteoblasts could be seen.Conclusion Parathyroid hormone combined with calcitonin mediated the expression of related bone growth factors,increased bone mineral density and mineral content of osteoporotic fractures,improved bone histomorphology and accelerated fracture healing.