异丙酚、氯胺酮对大鼠突触体Na~ ,K~ -ATP酶活性的影响

目的观察异丙酚、氯胺酮对大鼠不同脑区突触体Na ,K -ATP酶活性的影响。方法SD大鼠 ,随机分为三组 ,每组10只 ,分别ip生理盐水10ml/kg(对照组) ,异丙酚100mg/kg 或氯胺酮100mg/kg。用分光光度法测定大脑皮层、海马和脑干突触体Na ,K -ATP酶活性。结果与对照组比较 ,异丙酚明显抑制大脑皮层、海马和脑干突触体Na ,K -ATP酶活性(P<0.01),氯胺酮明显降低大脑皮层和脑干突触体Na ,K -ATP酶活性(P<0.01) ,但对海马突触体Na ,K -ATP酶活性无明显影响(P<0.01)。结论异丙酚、氯胺酮的全身麻醉作用可能与其抑制脑突触体Na ,K -ATP酶活性有关

Effects of propofol and ketamine on Na~ ,K~ -ATPase activity of synaptosomes in rat brain in vivo

Aim To investigate the effects of propofol and ketamine on Na , K -ATPase activity in rat brain synaptosomes. Methods Thirty SD rats were divided randomly into three groups. The aminals were given intraperitoneally (ip) normal saline 10ml/kg (control group), propofol 100mg/kg or ketamine 100mg/kg, respectively.Na ,K -ATPase activity of synaptosmes was assassed by spectrophotometric analysis. Results In the propofol group Na , K -ATPase activities of cerebrocortical, hippocampal and brain stem synaptosomes were significantly inhibited as compared with those in the control group (P<0.01).In the ketamine group, Na , K -ATPase activities of cerebrocortical and brain stem synaptosomes were significantly lower than those in the control group (P<0.01). Conclusions The general anesthesia effects of propofol and ketamine may be related to the inhibition of Na ,K -ATPase activity of brain synaptosomes in rats.