| 反相高效液相色谱法测定人血清中万古霉素的浓度 |
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| 引用本文： | 季闽春,薛杨勇,唐建国,杨春辉,杨耀芳. 反相高效液相色谱法测定人血清中万古霉素的浓度[J]. 中国临床药理学杂志, 2009, 25(4) |
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| 作者姓名： | 季闽春 薛杨勇 唐建国 杨春辉 杨耀芳 |
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| 作者单位： | 1. 复旦大学附属上海市第五人民医院,药剂科,上海,200240
2. 复旦大学附属上海市第五人民医院,重症监护中心,上海,200240 |
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| 摘 要： | 目的 建立反相高效液相色谱法测定人血清中万古霉素(糖肽类抗菌药)的浓度.方法 色谱柱为Shimadzu Shim-pack VP-ODS柱,流动相为甲醇-乙腈-0.05 mol·L-1磷酸二氢钾缓冲液(pH 3.2)=6.5:8.5:85,流量0.8 mL·min-1,紫外检测波长236 nm;盐酸去甲万古霉素为内标.结果 血清万古霉素在1-100 mg·L-1内线性良好,回归方程Y=24.09x-0.08(n=7,y=0.9993),日内和日间精密度RSD<10%.结论 该测定方法可靠,可用于临床血清万古霉素的浓度监测.
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| 关 键 词： | 反相高效液相色谱法 万古霉素 治疗药物监测 |
Determination of vancomycin concentration in human serum by RP-HPLC |
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| JI Min-chun,XUE Yang-yong,TANG Jian-guo,YANG Chun-hui,YANG Yao-fang. Determination of vancomycin concentration in human serum by RP-HPLC[J]. The Chinese Journal of Clinical Pharmacology, 2009, 25(4) |
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| Authors: | JI Min-chun XUE Yang-yong TANG Jian-guo YANG Chun-hui YANG Yao-fang |
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| Affiliation: | 1.Department of Pharmacy;2.General Intensive Care Unit;Shanghai The Fifth People's Hospital;Fudan University;Shanghai 200240;China |
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| Abstract: | Objective To establish a method of determining serum vancomycin in human by RP - HPLC. Methods Separation was car-ried out on a Shimadzu Shim -pack VP -ODS column, the mobile phase consisted of methanol, acetonitrile and buffer ( 6.5:8.5:85 ),flow rate was 0.8 mL·min-1 , UV detector wavelength was 236 nm. Norvancomycin was used as internal standard. Results A good lineari-ty was demonstrated between 1-100 mg·L-1 by linear equation y =24.09x - 0.08 (n=7,γ= 0.9993 ). The intra - day and inter - day deviation was showed by RSD < 10%. Consulsion The method is val-idated for determination of serum vancomyc in clinical practice. |
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| Keywords: | RP-HPLC vancomycin therapeutic drug monitoring |
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