RP—HPLC测定复方感冒止咳颗粒中绿原酸含量

目的 建立反相高效液相色谱法测定复方感冒止咳颗粒中绿原酸含量。方法 采用Shim-pack VP-ODS C18反相色谱柱（4．6mm×250mm，5μm），以乙腈-0．2％磷酸溶液（12：88）为流动相；流速为1．0mL·min^-1；检测波长为327nm；柱温：30℃。结果 绿原酸对照品进样量在0．101-0．805μg范围内线性关系良好（r=0．9999，n=5），平均加样回收率为99．67％，RSD为1．02％（n=6）。结论 方法简便快捷，结果准确可靠，可作于该制剂的含量测定。

Determination of chlorogenic acid in Fufang Ganmao Zhike granules by RP-HPLC

OBJECTIVE To establish a RP-HPLC method for the determination of chlorogenic acid in Fufang Ganmao Zhike granules. METHODS The chromatographic column of Shim-pack VP-ODS C18 （4.6 mm× 250ram, 5μm ）was used, the mobile phase consisting of acetonitrile and 0.2% （V/V）H3PO4 solution（12 ： 88, V/V）;The flow rate was 1.0mL·min^-1;the detection wavelength was set at 327 nm and the column temperature was maintained at 30℃. RESULTS Chlorogenic acid showed good linear relationship in the range of the 0.101 0.805 μg（r=0.999.9, n=5）,theaveragerecovery was 99.67%,RSD was 1.02%（n=6）.CONCLUSION The method is simple, quick, accurate and reliable, and can be used for the determination of this Preparation.