人成釉细胞瘤细胞的体外培养研究

目的 建立较稳定的人成釉细胞瘤细胞体外培养体系。方法 取成釉细胞瘤新鲜组织,应用DMEM培养基对成釉细胞瘤细胞进行原代组织块培养、传代培养及细胞纯化,并行超微结构观察及角蛋白、波形蛋白免疫组化标记。结果 细胞可连续传4代,成活50～60天;细胞多边形,呈铺路石状排列;电镜下见细胞间桥粒和胞内束状张力丝等上皮特征性超微结构;免疫组化标记:角蛋白阳性,波形蛋白阴性。结论 在此培养体系中,可成功地进行人成釉细胞瘤细胞体外连续培养。

CULTURE OF HUMAN AMELOBLASTOMA CELL IN VITRO

ve To establish a method of culturing human ameloblastoma cell. Methods Human fresh ameloblastoma tissue was cultured in DMEM complete culture medium (containing 10% fetal bovine serum, insulin and glutamine) . The cell growth behavior and morphology were observed by means of light microscopy and electron microscopy, and the expression of cytokeratin and vimentin was examined by immunohistochemistry. Results The cells could be maintained in culture up to 4 subcultures, or 50 - 60 days. Light microscopically, these cells had a polygonal shape, demonstrated typical intercellular bridges and arranged as a pavement-pattern. Ultrastructurally, the desmosomes and tonofila-ments confirmed the epithelial characteristic of cells. The cells showed positive staining for cytokeratin and negative for vimentin . Conclusion The culture method for growing human ameloblastoma cell has been established .