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| 同种异体大鼠骨髓间充质干细胞移植对放射性空肠损伤的修复作用 | |
| 引用本文： | 宋祥福,孙冰雪,张静春,龚守良. 同种异体大鼠骨髓间充质干细胞移植对放射性空肠损伤的修复作用[J]. 中国比较医学杂志, 2011, 21(8): 63-65,81. DOI: 10.3969/j.issn.1671-7856.2011.08.016 |
| 作者姓名： | 宋祥福 孙冰雪 张静春 龚守良 |
| 作者单位： | 1. 吉林大学公共卫生学院卫生部放射生物学重点实验室,长春,130021 2. 吉林大学生命科学院药物制剂,长春,130021 3. 吉林大学中日联谊医院检验科,长春,130033 |
| 摘 要： | 目的观察同种异体骨髓间充质干细胞(MSCs)移植对放射性空肠的修复作用。方法全骨髓贴壁体外培养大鼠MSCs并进行DAPI标记。对大鼠行5 Gy X线全腹部照射,每隔72 h照射1次,共5次,制备放射性空肠损伤动物模型。取40只大鼠,随机分成正常对照组、模型及MSCs治疗组及DAP标记组。激光共聚焦显微镜下观察DAPI标记的MSCs在空肠组织中富集情况。病理学观察MSCs对放射性空肠损伤的修复作用。结果正常大鼠空肠黏膜结构清楚,隐窝深遂,腺体丰富,模型组7 d黏膜上皮细胞坏死脱落,隐窝几乎完全破坏,治疗组7 d黏膜坏死组织较少,黏膜增厚,30 d治疗组核分裂相增加,较模型组增加明显。结论成功建立放射性空肠损伤动物模型,MSCs可以促进空肠再生与修复。 |
| 关 键 词： | 骨髓间充质干细胞 辐射 空肠 组织修复 |
Repairing Effect of Allogeneic Rat Bone Marrow Mesenchymal Stem Cells on Jejunal Radiation Damage |
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| SONG Xiang-fu,SUN Bing-xue,ZHANG Jing-chun,GONG Shou-liang. Repairing Effect of Allogeneic Rat Bone Marrow Mesenchymal Stem Cells on Jejunal Radiation Damage[J]. Chinese Journal of Comparative Medicine, 2011, 21(8): 63-65,81. DOI: 10.3969/j.issn.1671-7856.2011.08.016 | |
| Authors: | SONG Xiang-fu SUN Bing-xue ZHANG Jing-chun GONG Shou-liang |
| Affiliation: | SONG Xiang-fu1,SUN Bing-xue2,ZHANG Jing-chun3,GONG Shou-liang1(1.Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,2.School of Life Sciences,3.China-Japan Union Hospital of Jilin University,Changchun 130033) |
| Abstract: | Objective To observe the repairing effects of allogeneic bone marrow mesenchymal stem cells(MSCs) on jejunal radiation damage in rats.Methods Rat MSCs were isolated,purified and cultured in vitro.The model of ileal radiation damage was made.Sixty rats were randomly divided into normal control group,model group,and MSCs group(n=12).The enrichment of MSCs in the jejunum were observed under laser scanning confocal microscope.Pathological changes of the jejunum were examined.Results The structure of normal rat ... |
| Keywords: | Bone marrow mesenchymal stem cells Radiation Jejunum Tissue repair Rat |
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