| 紫外分光光度法测定金不换不同部位总黄酮含量 |
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| 引用本文： | 刘广河,丁艳霞,李亚真.紫外分光光度法测定金不换不同部位总黄酮含量[J].河南大学学报(医学版),2012(1):15-17. |
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| 作者姓名： | 刘广河 丁艳霞 李亚真 |
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| 作者单位： | 河南大学药学院中药教研室 |
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| 基金项目： | 河南大学教改项目(2009212) |
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| 摘 要： | 目的测定药用植物金不换不同部位总黄酮的含量。方法以体积分数为50%甲醇提取金不换不同部位的总黄酮,用紫外分光光度法测定其总黄酮含量。结果芦丁含量在0.031 35～0.073 15g/L范围内,线性关系良好,加样回收率100.5%,RSD为2.28%。结论金不换的根、茎、叶中总黄酮的含量均有不同,其中根的总黄酮含量明显高于叶和茎。
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| 关 键 词： | 金不换 紫外分光光度法 总黄酮 |
Determination of total flavonoids in different parts of Rumex obtusifolius L. by UV spectrophotometry |
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| LIU Guang-he,DING Yan-xia,LI Ya-zhen.Determination of total flavonoids in different parts of Rumex obtusifolius L. by UV spectrophotometry[J].Journal of Henan University,2012(1):15-17. |
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| Authors: | LIU Guang-he DING Yan-xia LI Ya-zhen |
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| Institution: | (Teaching Section of Chinese Materia,Pharmaceutical College of Henan University,Kaifeng,Henan 475004,China) |
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| Abstract: | Objective To study the total flavonoids contents in different parts of Rumex obtusifolius L.Methods Total flavonoids were extracted by 50% methanol in different parts of Rumex obtusifolius L.,UV spectrophotometry was selected to determine the contents.Results The total flavonoids contents was at the range of 0.031 35~0.073 15 g/L,the linearity was good,the average recoveries were 100.5%,with RSD=2.28%.Conclusion The roots,stems and leaves contain different flavonoids.The total flavonoid contents of root and stems are significantly higher than that of leaves. |
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| Keywords: | Rumex obtusifolius L UV spectrophotometry Total flavonoids |
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