急性甲醇中毒大鼠空间学习记忆功能改变的研究

目的 探讨大鼠急性高剂量和低剂量甲醇中毒后空间学习记忆功能的改变.方法 复制急性高剂量和低剂量甲醇中毒大鼠各10只,并设生理盐水对照组(n=10),给予各组大鼠灌胃相应剂量甲醇溶液或生理盐水,并吸入等比(1∶1)混合气体(N20/02);应用Morris水迷宫评价急性甲醇中毒大鼠的空间学习记忆功能.结果 Morris水迷宫结果显示:与生理盐水对照组(60.43±7.13)s相比,低剂量组(89.55±7.98)s和高剂量组(98.77±5.64)s在Morris水迷宫水下隐匿平台实验第1天的逃避潜伏期显著延长(P＜0.05)；第2～5天高剂量组逃避潜伏期较低剂量组和生理盐水对照组明显延长(P＜0.05)；3组在第6～7天的水上可见平台实验中的逃避潜伏期时间和无平台探测实验中第Ⅲ象限的停留时间均无显著性差异(P＞0.05).结论 急性甲醇中毒大鼠有空间学习记忆功能障碍,程度与甲醇中毒剂量有关.

A Study on the Spatial Learning and Memory Ability after Acute Methanol Poisoning in Rats

Objective To study the change of spatial learning and memory fuction in rats induced by acute methanol poisoning.Methods Animal model of methanol poisoning was made,and rats received normal saline were selected as control.The rats were placed in a plexiglas chamber that exposed to a mixture of N2O/O2and gaved different dosage of methanol by gavage.The spatial learning and memory ability was assessed with Morris Water Maze.Results Compared with the control group(60.43±7.13) s,the performance latency on the Morris Water Maze prolonged in low dosage group(89.55±7.98) s and high dosage group(98.77±5.64) s rats on the first day post-injury.The latency in high dosage group was much longer than that in low dosage group and control group on the 2nd~5th day post-injury(P <0.05).There were no statistical differences among three groups during the visible platform tests and probe tests.Conclusion Rats subjected to acute methanol poisoning have spatial learning and memory function dysfunction and,in this connection,the degree of poisoning and the dose administered is apparently related.