Bcl-2基因在一氧化碳暴露大鼠脑中的表达

目的 观察一氧化碳(CO)暴露后大鼠脑中Bcl-2基因的分布。方法 用Wistar大鼠制成CO暴露动物模型，随机分组，分别在染毒后第3天、第2天和当天处死，取脑制成切片，用地高辛Bcl-2探针原位杂交，观察Bcl-2的信号分布。结果 Bcl-2 mRNA的表达在海马、大脑皮层和胼胝体比较明显，其中海马的表达信号最强，大脑皮层次之，胼胝体最弱。其他部位呈散在的弱信号。相应区域在各个时间点无明显的差异。对照组未见表达。结论 CO暴露后，Bcl-2在海马、大脑皮层和胼胝体的表达较为明显；Bcl-2在上述部位的表达可能部分的解释CO中毒迟发性神经损伤的发生。

The expression of Bcl-2 in the brain of Wistar rats after carbon monoxide exposure

Objective To observe the expression of Bcl-2 mRNA in the brain of Wistar rats after carbon monoxide exposure.Methods The model of CO exposure was made with Wistar rats,and they were randomly divided into four groups.Group 1,group 2,and group 3 were separately put in the air after they were respectively killed on the third,second and first day after CO exposure.Group 4 was the control group.The expressions of Bcl-2 gene in rat brains were studied by using in situ hybridization with Bcl-2 mRNA probe.Results The results revealed that the expression of Bcl-2 mRNA was obvious in hippocampus,cerebral cortex and corpus callosum after CO exposure.The signal of hybridization in hippocampus was the strongest.There was no marked expression in the control group.The expressions of the gene was not significantly different in various time points and in group 1,2,3.Conclusions The expression of Bcl-2 mainly occurs in hippocampus,cerebral cortex and corpus callosum after CO exposure.The expression of Bcl-2 in the above areas may partly acount for the development of secondary brain damage after CO exposure.