氯沙坦对肾缺血再灌注大鼠肾组织超氧化物歧化酶和丙二醛的影响

目的 :探讨氯沙坦干预急性肾缺血再灌注损伤的保护机制。方法 :实验大鼠分正常组、假手术组、缺血再灌注模型组、氯沙坦组 (预先喂服氯沙坦 )。检测各组在急性肾缺血 1h、再灌注 1h和 2 4h肾组织匀浆中超氧化物歧化酶 (SOD)活力与丙二醛 (MDA )含量变化。结果 :模型组和氯沙坦组MDA含量与正常组和假手术组比较均显著增高 (P<0 .0 1) ;氯沙坦组在再灌注 1h和 2 4h ,MDA含量(7.7± 1.0 )nmol·mg- 1pro和 (9.2± 0 .5 )nmol·mg- 1pro较模型组 (9.5± 0 .4 )nmol·mg- 1pro和(11.9± 0 .9)nmol·mg- 1pro明显降低 (P <0 .0 5 )。模型组与正常组和假手术组比较SOD活力明显降低 (P <0 .0 5 ) ;氯沙坦组在缺血 1h ,再灌注 1h和2 4h ,SOD活力分别为 (81.3± 1.6 )NU·mg- 1pro ,(79± 4 )NU·mg- 1pro和 (48± 4 )NU·mg- 1pro)较模型组 (6 8± 8)NU·mg- 1pro ,(48± 11)NU·mg- 1pro和 (18± 4 )NU·mg- 1pro明显增高 (P <0 .0 5 )。结论 :氯沙坦能减轻急性肾缺血再灌注时氧自由基引起的损伤

Effect of losartan on renal tissue levels of superoxide dismutase and malondialdehyde in rats with renal ischemia-reperfusion injury

AIM: To investigate the protective effects of losartan on renal ischemia-reperfusion injury in rats. METHODS: Rats were divided into four groups: normal control, sham-operation, renal ischemia-reperfusion model and losartan groups. Losartan was fed by ig to the rats as early as the day two weeks before renal ischemia-reperfusion. After 1 h renal ischemia, 1 h and 24 h renal ischemia-reperfusion, the content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were detected in the homogenate of renal tissues. RESULTS: Compared with normal control and sham-operation groups, the MDA content in model, losartan groups were significantly higher (P<0.01) and SOD activity in model group was profoundly lower (P<0.05). After 1 h and 24 h reperfusion, the MDA content in losartan group lowered from (9.5±0.4) nmol·mg -1pro and (11.9±0.9) nmol·mg -1pro to (7.7±1.0) nmol·mg -1pro and (9.2± 0.5) nmol·mg -1 pro. Compared with model group and SOD activity in losartan group compared with model group in creased from (48±11) NU·mg -1pro and (18±4) to (79±4) NU·mg -1pro and (48±4) NU·mg -1 pro(P<0.05). CONCLUSION: Losartan can relieve the damage caused by activated oxygen free redical increased significantly in acute renal ischemia-reperfusion rats.