国产氧化型染发剂对小鼠皮肤的亚慢性毒作用

目的 ]研究氧化型染发剂经皮染毒对小鼠的亚慢性毒作用。 方法 ]以市售氧化型染发剂对小鼠背部皮肤染毒 ,每天一次 ,持续 3 0d。观察小鼠的一般生长情况、血液生化指标及肝、肾、肺、脾和皮肤组织病理学改变及遗传毒性。 结果 ]随着氧化型染发剂染毒剂量的增加 ,与对照组相比 ,高剂量组的体重增长显著减缓 ;高剂量组小鼠的血清谷丙转氨酶 (ALT)、碱性磷酸酶 (ALP)、血清尿素氮 (BUN)和肌酐 (CR)值显著增高 ,肝、肺重量显著降低 ,肾重量显著增高 (P<0 .0 5或P <0 .0 1)。病理组织学检查显示 ,高剂量组生发上皮细胞和皮脂腺细胞大量细胞核固缩 ,毛囊四周生发细胞数量减少 ,真皮组织炎性细胞浸润明显 ,与对照组和低剂量组相比 ,毛囊数量显著减少。同时骨髓嗜多染红细胞微核率增高 ,并观察到表皮细胞的DNA损伤。 结论 ]在亚慢性染毒条件下 ,氧化型染发剂可影响小鼠肝、肾功能 ,并具有潜在的遗传毒性

Subchronic Toxicity of Oxidized Hair Dyes to Mice

Objective To study the subchronic toxicity of the oxidized hair dyes in mice.Methods] Commercial oxidized hair dyes were applied onto the skin of mice once everyday for a month.During the testing period,biological effects were observed,including body weight,biochemical examination of blood,histopathology of skin,liver,spleen,lung and kidney,and bone marrow genotoxicity.Results] As the dose increased,the blood biochemical indicators(ALT,ALP,BUN and CR) changed significantly,the weight of body and organs(liver,lung)decreased but increased for kidney.Alteration of histopathology showed that in high dose group many nuclei pyknosed in germinal cells and sebaceous gland cells, germinal cells of peri-hair follicles decreased,inflammatory cells infiltrated in coriun tissue.Compared to control and low dose group,the hair follicles decreased significantly.The DNA strand breaks were observed in the mouse bone marrow cells and epidermic cells. Conclusion] The results suggested that potential genotoxic risks of the oxidized hair dyes could not be ruled out.