姜黄素对IGF-1调控的宫颈癌淋巴转移的影响

目的:研究姜黄素对IGF-1调控的宫颈癌淋巴转移的影响。方法:运用实时荧光定量PCR检测IGF-1不同浓度、作用时间以及姜黄素作用下宫颈癌siha细胞中VEGF-C mRNA的表达。结果:①宫颈癌siha细胞VEGF-C mRNA的表达随IGF-1浓度的递增而变化,10ng/ml时达峰值。0.5ng/ml1、ng/ml、5ng/ml、10 ng/ml、50 ng/ml组VEGF-C mRNA表达量依次为对照组的1.34、1.67、3.14、5.28和3.02倍。5ng/ml(6.73±0.25)1、0 ng/ml(5.98±0.18)、50 ng/ml(6.79±0.12)组较对照组差异具有统计学意义(P<0.05)。②宫颈癌siha细胞VEGF-C mRNA表达随IGF-1不同作用时点而变化,6h达峰值。2h组、4h组、6h组和8h组的表达量分别为对照组的1.30、2.51、5.33和2.83倍。4h(6.80±0.12)、6h(5.66±0.24)和8h组(6.03±0.10)较对照组差异具有统计学意义(P<0.05)。③IGF-1I、GF-1+Cur(10μm/ml)和IGF-1+Cur(20μm/ml)组VEGF-C mRNA的表达量依次为对照组的5.51、1.42和0.67倍。IGF-1组(7.20±0.85)I、GF-1+Cur(20μm/ml)(10.23±1.28)的表达量较对照组差异具有统计学意义。IGF-1+Cur(10μm/ml)(9.15±0.47)和IGF-1+Cur(20μm/ml)(10.23±1.28)组的表达量与IGF-1组差异具有统计学意义。IGF-1+Cur(20μm/ml)(10.23±1.28)的表达量与对照组差异亦具有统计学意义。结论:姜黄素能有效降低VEGF-C的表达,显著抑制IGF-1调控的宫颈癌淋巴转移。

Effects of Curcumin on the Lymphatic Metastasis Regulated by IGF-1 in Human Uterine Cervical Cancer

Abstract: Objective: To study the effects of curcumin on the lymphatic metastasis regulated by IGF-1 in human uterine cervical cancer.Methods: The expression of VEGF-C mRNA in siha cell line was detected by real-time PCR with administration of IGF-1 at different concentrations and time points and curcumin.Results: ①The expression of VEGF-C mRNA varied according to concentrations of IGF-1 and reached the peak at 10ng/ml.The expression of group 0.5ng/ml,1 ng/ml,5ng/ml,10ng/ml and 50ng/ml were 1.34,1.67,3.14,5.28 and 3.02 times higher than that in the control group.The expression of 5ng/ml(6.73±0.25),10ng/ml(5.98±0.18) and 50ng/ml(6.79±0.12) group were significantly higher than that in the control(P<0.05).②The expression of VEGF-C mRNA differed at various time points and reached the peak at 6h.The expression of group 2h,4h,6h and 8h were 1.30,2.51,5.33 and 2.83 times higher than the control group.Group 4h,6h and 8h were significantly larger than that in the control group(P<0.05).③ The expression of VEGF-C mRNA of group IGF-1,IGF-1+Cur(10μm/ml)and IGF-1+Cur(20μm/ml)were 5.51,1.42 and 0.67 times higher than the control group.The relative expression of IGF-1 and IGF-1 +Cur(20μm/ml)group were 7.20 ± 0.85 and 10.23 ±1.28,showing significant difference with that in the control group There were also significant differences between IGF-1 +Cur(10μm/ml),IGF-1+Cur(20μm/ml)group and IGF-1 group and(P<0.05).Conclusion: curcumin can effectively decrease the expression of VEGF-C and inhibit the lymphatic metastasis regulated by IGF-1.